1 MISEV2023: DELIVERING THE GOODS

MISEV2023 has been written and revised to stand on its own as a guide to EV studies . Starting with an introduction with a historical perspective and ‘user guide’, MISEV2023 next takes the reader through all of the main aspects of an EV study. Community-sourced, it has something to offer beginners and veterans alike. The nomenclature section introduces the diversity of EVs and the need to avoid misusing the term ‘exosome’. It also highlights the ‘non-vesicular extracellular particles’ (NVEPs) that abound in most EV preparations. Collection and pre-processing underscores the importance of pre-analytical variables, with input on specific EV sources from ISEV task forces. EV separation and concentration and EV characterization have new details on key approaches. A new section on technique-specific reporting for EV characterization focuses on leading commercially available methods. EV release and uptake examines opportunities and pitfalls, while functional studies reminds us of important controls that are needed to help attribute an outcome to EVS. Finally, there is a new section on studying EVs in vivo.

中文翻译：

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MISEV2023：电动汽车研究和应用更新指南

1 MISEV2023：交付货物

MISEV2023 已经过编写和修订，可以独立作为 EV 研究指南。MISEV2023 首先从历史角度和“用户指南”进行介绍，然后带领读者了解电动汽车研究的所有主要方面。它源自社区，可以为初学者和老手提供一些东西。命名部分介绍了 EV 的多样性以及避免滥用术语“外泌体”的必要性。它还强调了大多数 EV 制剂中富含的“非囊泡细胞外颗粒”(NVEP)。收集和预处理强调了预分析变量的重要性，并提供了 ISEV 工作组对特定 EV 来源的输入。EV 分离和集中以及EV 表征在关键方法上有新的细节。关于 EV 表征的特定技术报告的新部分重点关注领先的商业可用方法。EV 的释放和吸收检查机会和陷阱，而功能研究提醒我们需要重要的控制措施来帮助将结果归因于 EVS。最后，有一个关于体内电动汽车研究的新部分。