PurposeTo observe the inhibitory effect of the small molecule tyrosine kinase inhibitor apatinib on small cell lung cancer in vitro and vivo.Material and methodsThree small lung cancer cells were selected CCK‐8 and monoclonal assay was used to determine the effect of apatinib on proliferation. The effects on cell cycle and apoptosis were detected by flow cytometry and TUNEL. We observed the inhibitory effect of different doses of apatinib on xenograft tumor. The efficacy and safety of apatinib in 20 patients with advanced small cell lung cancer were observed.ResultsFor small cell lung cancer with high expression of VEGFR2, apatinib has a significant inhibitory effect both in vitro and in vivo. It can play an inhibitory role by promoting apoptosis and cell cycle arrest pathways. For small cell lung cancer with low expression of VEGFR, the inhibitory effect on cells in vitro was not significant. It has certain inhibitory effect on nude mouse transplanted tumor and small cell lung cancer patients, but the effect is weaker than that of animal models and patients with small cell lung cancer cells with high expression of VEGFR2.ConclusionApatinib has a significant inhibitory effect on small cell lung cancer with high expression of VEGFR2 and may be a treatment for small cell lung cancer patients.

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阿帕替尼对不同小细胞肺癌细胞以及肺癌荷瘤小鼠和患者的抑制作用

目的观察小分子酪氨酸激酶抑制剂阿帕替尼对小细胞肺癌的体内外抑制作用。材料与方法选取CCK-8细胞3株，单克隆实验测定阿帕替尼对增殖的影响。通过流式细胞术和TUNEL检测对细胞周期和凋亡的影响。我们观察了不同剂量的阿帕替尼对异种移植瘤的抑制作用。观察阿帕替尼治疗20例晚期小细胞肺癌的疗效和安全性。结果对于VEGFR2高表达的小细胞肺癌，阿帕替尼在体外和体内均具有显着的抑制作用。它可以通过促进细胞凋亡和细胞周期阻滞途径发挥抑制作用。对于VEGFR低表达的小细胞肺癌，体外对细胞的抑制作用不显着。对裸鼠移植瘤和小细胞肺癌患者有一定的抑制作用，但效果弱于动物模型和高表达VEGFR2的小细胞肺癌细胞患者。结论阿帕替尼对小细胞有显着的抑制作用高表达VEGFR2的肺癌可能成为小细胞肺癌患者的治疗方法。