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Performance assessment of a new G12/A1 antibody-based rapid ELISA using commercially available and gluten-spiked food samples.
European Journal of Gastroenterology & Hepatology ( IF 2.1 ) Pub Date : 2024-02-21 , DOI: 10.1097/meg.0000000000002739
Anil K. Verma 1, 2 , Chiara Monachesi 2 , Giulia N. Catassi 3 , Elisa Franceschini 3 , Simona Gatti 3 , Elena Lionetti 3 , Carlo Catassi 3, 4
Affiliation  

Food products with <20 mg/kg gluten can be labeled 'gluten-free' according to international regulations. Several antibodies-based ELISAs have been develop to track gluten traces in food products. Among them, R5 and G12 antibody-based ELISAs are the frequently used methods. However, these antibodies have certain limitations. We evaluated the accuracy of G12/A1 antibody-based 'Glutentox ELISA Rapid G12' and compared the results with the current reference method i.e., R5 antibody-based 'Ridascreen R5 ELISA'.

中文翻译:

使用市售的麸质食品样品对基于 G12/A1 抗体的新型快速 ELISA 进行性能评估。

根据国际法规,麸质含量<20 mg/kg 的食品可贴上“无麸质”标签。几种基于抗体的 ELISA 已被开发用于追踪食品中的麸质痕迹。其中,基于R5和G12抗体的ELISA是常用的方法。然而,这些抗体有一定的局限性。我们评估了基于 G12/A1 抗体的“Glutentox ELISA Rapid G12”的准确性,并将结果与​​当前参考方法(即基于 R5 抗体的“Ridascreen R5 ELISA”)进行了比较。
更新日期:2024-02-21
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