β‐Catenin (CTNNB) is a core downstream mediator in the Wnt/β‐catenin signaling pathway, which plays a role in immune regulation and cell differentiation. In the present study, two CTNNB genes were identified by genomic analysis of Hippocampus erectus and named He‐CTNNB1 and He‐CTNNB2. He‐CTNNB1 was more conserved than He‐CTNNB2. The full length of He‐CTNNB1 was 2369 bp, including an open reading frame of 2275 bp that encoded 737 amino acids containing 12 armadillo repeats. Phylogenetic analysis revealed that He‐CTNNB1 clusters had homologs in other bony fishes. Tissue distribution analysis showed the highest mRNA expression of He‐CTNNB1 in the ovary, liver, and kidney of the lined seahorse under normal physiological conditions. The transcription level of He‐CTNNB1 was significantly increased in the liver and kidney in response to LPS, poly I:C, and Edwardsiella tarda challenge (p < 0.05). In vitro overexpression of He‐CTNNB1 significantly upregulated the expression of key genes of the canonical Wnt/β‐catenin signaling pathway, such as axin2, cMyc, and cyclinD (p < 0.05), which increased cell proliferation during the wound‐healing process. Taken together, our results suggest that He‐CTNNB1 is essential for cell proliferation and the innate immune response in H. erectus.

中文翻译：

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直立海马中 CTNNB1 的鉴定及其在先天免疫和细胞增殖中的功能

β-连环蛋白（CTNNB）是Wnt/β-catenin信号通路中的核心下游介质，在免疫调节和细胞分化中发挥作用。在本研究中，两个CTNNB通过基因组分析鉴定了基因直立海马并命名为He-CTNNB1和He-CTNNB2。He-CTNNB1比更保守He-CTNNB2。全长为He-CTNNB1该基因全长2369 bp，包括2275 bp的开放阅读框，编码737个氨基酸，含有12个犰狳重复序列。系统发育分析表明He-CTNNB1簇在其他硬骨鱼中具有同源物。组织分布分析显示 mRNA 表达量最高He-CTNNB1正常生理条件下，存在于有纹海马的卵巢、肝脏和肾脏中。转录水平为He-CTNNB1LPS、poly I:C 和多聚 I:C 对肝脏和肾脏的反应显着增加迟缓爱德华氏菌挑战 （p< 0.05）。体外过度表达He-CTNNB1显着上调经典 Wnt/β-catenin 信号通路关键基因的表达，例如轴心2,cMyc， 和细胞周期蛋白D（p< 0.05），这增加了伤口愈合过程中的细胞增殖。综上所述，我们的结果表明He-CTNNB1对于细胞增殖和先天免疫反应至关重要直立人。