CAPA neuropeptides regulate the diuresis/ antidiuresis process in insects by activating specific cognate receptor, . In this study, we characterized the gene () in the whitefly, Asia II 1. The two alternatively spliced isoforms of gene, -1 and -2, having six and five exons, respectively, were identified. The gene expression was highest in adult whitefly as compared to gene expression in egg, nymphal and pupal stages. Among the three putative CAPA peptides, CAPA-PVK1 and CAPA-PVK2 strongly activated the -1 with very low EC values of 0.067 nM and 0.053 nM, respectively, in heterologous calcium mobilization assays. None of the peptide activated the alternatively spliced isoform -2 that has lost the transmembrane segments 3 and 4. Significant levels of mortality were observed when whiteflies were fed with CAPA-PVK1 at 1.0 μM (50.0%), CAPA-PVK2 at 100.0 nM (43.8%) and CAPA-tryptoPK 1.0 μM (40.0%) at the 96 h after the treatment. This study provides valuable information to design biostable peptides to develop a class of insecticides.

中文翻译：

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功能测定确定了烟粉虱 (Gennadius) 中 CAPA 肽和 CAPA 受体亚型之间的真实相互作用

CAPA 神经肽通过激活特定的同源受体来调节昆虫的利尿/抗利尿过程。在本研究中，我们鉴定了粉虱 Asia II 1 中的基因 ()。鉴定了基因的两个选择性剪​​接亚型 -1 和 -2，分别具有 6 个和 5 个外显子。与卵、若虫和蛹阶段的基因表达相比，成年粉虱的基因表达最高。在三种假定的 CAPA 肽中，CAPA-PVK1 和 CAPA-PVK2 在异源钙动员测定中强烈激活 -1，EC 值分别非常低，分别为 0.067 nM 和 0.053 nM。没有肽激活已丢失跨膜片段 3 和 4 的选择性剪接亚型 -2。当粉虱喂食 1.0 μM (50.0%) 的 CAPA-PVK1 和 100.0 nM (100.0 nM) 的 CAPA-PVK2 时，观察到显着的死亡率水平。 43.8%）和治疗后96小时CAPA-tryptoPK 1.0 μM（40.0%）。这项研究为设计生物稳定肽以开发一类杀虫剂提供了有价值的信息。