The present study successfully synthesized nano CoAlO by the solution combustion method, employing sucrose as an ergonomic fuel. The synthesis of CoAlO was confirmed by X-ray diffraction, Raman spectroscopy as well as electron microscopy, and a mean size of 70–80 nm was observed. Besides, the antioxidant, antimicrobial, photocatalytic and antibiofilm potentials of nano CoAlO were evaluated against multi-drug resistant (MDR) bacterial strains. Initially, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CoAlO (µg/mL) were determined to be within the ranges of 62.50–250 and 250–500, respectively. The poultry red blood cell-based hemolysis and secondary cell line-based MTT assays revealed concentration-dependent toxicity with an inhibitory concentration-50 (µg/mL) of 75.0 and 25.0 against MDA-MB468 and HT29 cell lines, respectively. Interestingly, CoAlO was found to generate reactive oxygen species and revealed potential antioxidant properties by DPPH and reducing power assays. Moreover, the synthesized materials permeabilized the membranes of both Gram-positive () and Gram-negative () bacteria and were found to be bactericidal and exhibited antibiofilm activity against the MDR- strain. The nano CoAlO, when treated with the MIC and 1/2X MIC, eliminated MDR-methicillin-resistant within 60 and 120 min, respectively, and was found to be an excellent photosensitizer. In short, this study concludes that CoAlO was found to be a promising antimicrobial candidate with antioxidant as well as antibiofilm properties for application in wastewater treatment and therapeutic purposes.

中文翻译：

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纳米钴铝氧化物的合成和表征作为潜在的抗氧化剂、杀菌剂和光催化消毒剂，对抗具有公共卫生意义的多重耐药病原体

本研究采用蔗糖作为符合人体工学的燃料，通过溶液燃烧法成功合成了纳米CoAl2O。通过 X 射线衍射、拉曼光谱以及电子显微镜证实了 CoAl2O 的合成，并观察到平均尺寸为 70-80 nm。此外，还针对多重耐药（MDR）菌株评估了纳米CoAl2O的抗氧化、抗菌、光催化和抗生物膜潜力。最初，CoAl2O(μg/mL)的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别确定在62.50-250和250-500的范围内。基于家禽红细胞的溶血和基于二次细胞系的 MTT 测定显示，对 MDA-MB468 和 HT29 细胞系具有浓度依赖性毒性，抑制浓度 50 (μg/mL) 分别为 75.0 和 25.0。有趣的是，CoAlO 被发现可以产生活性氧，并通过 DPPH 和还原力测定揭示了潜在的抗氧化特性。此外，合成材料可透化革兰氏阳性 () 和革兰氏阴性 () 细菌的膜，并被发现具有杀菌作用，并对 MDR 菌株表现出抗生物膜活性。当用 MIC 和 1/2X MIC 处理时，纳米 CoAlO 分别在 60 和 120 分钟内消除了 MDR 甲氧西林耐药性，并且被发现是一种优异的光敏剂。简而言之，本研究得出的结论是，CoAlO 被发现是一种有前景的抗菌候选药物，具有抗氧化和抗生物膜特性，可用于废水处理和治疗目的。