Tartary buckwheat protein (BWP) is well known for the wide-spectrum antibacterial activity and the lipid metabolism- regulating property; therefore, BWP can be applied as feed additives to improve the animal’s nutritional supply. With the aim to investigate the bioactive actions of the BWP, growth performance, lipid metabolism and systemic immunity of the weaned piglets were measured, and the alterations of pig gut microbiota were also analyzed. According to the results, the growth performances of the weaned piglets which were calculated as the average daily gain (ADG) and the average daily feed intake (ADFI) were significantly increased when compared to the control group. Simultaneously, the serum levels of the total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were decreased, while the levels of high-density lipoprotein cholesterol (HDL-C) were increased in the BWP group. Moreover, the relative abundances of Lactobacillus, Prevotella_9, Subdoligranulum, Blautia, and other potential probiotics in the gut microbiota of weaned piglets were obviously increased in the BWP group. However, the relative abundances of Escherichia-Shigella, Campylobacter, Rikenellaceae_RC9_gut_group and other opportunistic pathogens were obviously decreased in the BWP group. In all, BWP was proved to be able to significantly improve the growth performance, lipid metabolism, and systemic immunity of the weaned piglets, and the specific mechanism might relate to the alterations of the gut microbiota. Therefore, BWP could be explored as a prospective antibiotic alternative for pig feed additives.

苦荞蛋白（BWP）以其广谱抗菌活性和脂质代谢调节特性而闻名；因此，BWP可以作为饲料添加剂来改善动物的营养供给。为了研究BWP的生物活性，测量了断奶仔猪的生长性能、脂质代谢和全身免疫力，并分析了猪肠道微生物群的变化。结果显示，与对照组相比，断奶仔猪的生长性能（以平均日增重（ADG）和平均日采食量（ADFI）计算）显着提高。同时，血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平降低，而高密度脂蛋白胆固醇（HDL-C）水平升高。 BWP 组。此外，BWP组断奶仔猪肠道菌群中乳杆菌、普氏菌_9、Subdoligrinum、Blautia等潜在益生菌的相对丰度明显增加。而BWP组中大肠杆菌、弯曲杆菌、Rikenellaceae_RC9_gut_group等机会致病菌的相对丰度明显降低。综上所述，BWP被证明能够显着改善断奶仔猪的生长性能、脂质代谢和全身免疫力，其具体机制可能与肠道菌群的改变有关。因此，BWP 可以作为猪饲料添加剂的抗生素替代品进行探索。