Escherichia coli is a Gram-negative bacterium that prominently causes a variety of clinical infections in humans, such as diarrhea, sepsis, and urinary tract infection. This bacterium is a common multidrug-resistant threat in community and hospital settings worldwide. This study examined the antimicrobial susceptibility and genetic relationship based on Clermont phylotyping and ERIC-PCR of 84 E. coli urinary isolates from provincial and community hospitals in Thailand. All the isolates were completely susceptible to nitrofurantoin, whereas almost all isolates were susceptible to carbapenem, fosfomycin, and amikacin. A high resistance rate was found to fluoroquinolone, ampicillin, and trimethoprim/sulfamethoxazole. Clermont phylogroup B2 was predominant (n=58). Subtyping of the B2 phylogroup revealed diverse subgroups, of which subgroup V (n=11) was predominant, followed by VII (n=9), III (n=6), and II (n=6). ERIC-PCR showed the strain of the B2 subgroups III and V were spread between provincial and community hospitals and between hospital wards. This evidence suggested the need for comprehensive infection control monitoring, with strong active surveillance at all hospital levels.

大肠杆菌是一种革兰氏阴性细菌，主要引起人类多种临床感染，如腹泻、败血症和尿路感染。这种细菌是全球社区和医院环境中常见的多重耐药威胁。本研究基于克莱蒙系统分型和 ERIC-PCR，对来自泰国省级和社区医院的 84 份尿大肠杆菌分离株进行了抗菌药物敏感性和遗传关系的检测。所有分离株对呋喃妥因完全敏感，而几乎所有分离株对碳青霉烯、磷霉素和阿米卡星敏感。对氟喹诺酮类、氨苄西林、甲氧苄啶/磺胺甲恶唑耐药率较高。克莱蒙系统群 B2 占主导地位 (n=58)。B2 系统群的亚型显示了不同的亚群，其中 V 亚群 (n=11) 占主导地位，其次是 VII (n=9)、III (n=6) 和 II (n=6)。ERIC-PCR显示B2亚型III和V菌株在省级和社区医院以及医院病房之间传播。这一证据表明需要进行全面的感染控制监测，并在各级医院进行强有力的主动监测。