Abstract

As part of a long-term study aiming to isolate and identify yeast species that inhabit the surface of leaves and fruits of native fine-aroma cacao in the department of Amazonas, Peru, we obtained multiple isolates of Hannaella species. Yeasts of the genus Hannaella are common inhabitants of the phyllosphere of natural and crop plants. On the basis of morphological, and physiological characteristics, and sequence analysis of the D1/D2 domains of the large subunit rRNA gene (LSU) and the internal transcribed spacer region (ITS), we identified five species of Hannaella from the phyllosphere of Peruvian cacao. Four have been previously described: H. phyllophila (isolates KLG-073, KLG-091), H. pagnoccae (KLG-076), H. sinensis (KLG-121), and H. taiwanensis (KLG-021). A fifth, represented by eight isolates (KLG-034, KLG-063, KLG-074, KLG-078, KLG-79, KLG-082, KLG-084, KLG-085), is not conspecific with any previously described Hannaella species, and forms the sister clade to H. surugaensis in the phylogenetic analysis. It has 2.6–3.9% (18–27 substitutions, 2–4 deletions, and 1–3 insertions in 610–938 bp-long alignments), and 9.8–10.0% nucleotide differences (37 substitutions and 14 insertions in 511–520 bp-long alignments) in the LSU and ITS regions, respectively, to H. surugaensis type strain, CBS 9426. Herein, the new species Hannaella theobromatis sp. nov. is described and characterised. The species epithet refers to its epiphytic ecology on its host Theobroma cacao.

中文翻译：

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秘鲁可可叶和果实的表面是多种汉纳氏酵母菌的家园，其中包括新种汉纳氏菌theobromatis sp。十一月

摘要

作为一项长期研究的一部分，旨在分离和鉴定栖息在秘鲁亚马逊地区本地香型可可叶子和果实表面的酵母种类，我们获得了多种汉娜菌分离株。汉娜氏菌属酵母是天然植物和农作物叶际的常见居民。基于形态、生理特征以及大亚基 rRNA 基因 (LSU) 的 D1/D2 结构域和内转录间隔区 (ITS) 的序列分析，我们从秘鲁可可叶际中鉴定出 5 个汉娜菌属 (Hannaella)物种。。之前已描述过四种：H. phyllophila（分离株 KLG-073、KLG-091）、H. pagnoccae（KLG-076）、H. sinensis（KLG-121）和H. taiwanensis（KLG-021）。第五种以八个分离株（KLG-034、KLG-063、KLG-074、KLG-078、KLG-79、KLG-082、KLG-084、KLG-085）为代表，与任何先前描述的汉娜菌属物种均不相同，并在系统发育分析中形成H. surugaensis的姐妹进化枝。它有 2.6-3.9%（610-938 bp 长的比对中 18-27 个替换、2-4 个缺失和 1-3 个插入）和 9.8-10.0% 的核苷酸差异（511-520 bp 中的 37 个替换和 14 个插入）在 LSU 和 ITS 区域中分别与H. surugaensis型菌株 CBS 9426 进行长比对）。在此，新种Hannaella theobromatis sp. 十一月 被描述和表征。该物种的绰号是指其寄主可可上的附生生态。