This investigation focused on assessing and enhancing ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES) to extract phenolics and terpenoids from sweet basil leaves. The initial stage involved evaluating the extraction performance of twelve NADES and ethanol. A NADES comprising lactic acid and glucose with a 2 : 1 molar ratio and 20% water content (WC) obtained the highest total phenolic content (TPC) and total terpenoid content (TTC). Single-factor experiments systematically examined the impact of liquid-to-solid ratio (LSR), water content in NADES (WC), ultrasound power, temperature, and exposure time on the extraction yield. Optimization using Box–Behnken Design (BBD) models for the lactic acid and glucose-based UAE revealed the optimal conditions to be 80 ml/g LSR, 30% water, 300 W, 50°C temperature, and a 15-minute exposure time. Under these optimized parameters, the extraction achieved the highest TPC and TTC at 69.88 mg GAE/g and 110.71 mg UA/g, respectively. This study presents an environmentally friendly and sustainable extraction protocol for the extraction of phenolic compounds and terpenoids from sweet basil leaves.

中文翻译：

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使用天然低共熔溶剂超声辅助提取甜罗勒叶中酚类和萜类化合物的优化

本研究的重点是评估和增强超声波辅助提取 (UAE)，使用天然低共熔溶剂 (NADES) 从甜罗勒叶中提取酚类和萜类化合物。初始阶段涉及评估 12 种 NADES 和乙醇的提取性能。含有摩尔比为2:1的乳酸和葡萄糖且含水量(WC)为20%的NADES获得了最高的总酚含量(TPC)和总萜含量(TTC)。单因素实验系统地考察了液固比（LSR）、NADES 中的水含量（WC）、超声功率、温度和暴露时间对提取率的影响。使用 Box–Behnken Design (BBD) 模型对基于乳酸和葡萄糖的 UAE 进行优化，结果表明最佳条件为 80 ml/g LSR、30% 水、300 W、50°C 温度和 15 分钟暴露时间。在这些优化参数下，提取物获得了最高的 TPC 和 TTC，分别为 69.88 mg GAE/g 和 110.71 mg UA/g。本研究提出了一种环保且可持续的提取方案，用于从甜罗勒叶中提取酚类化合物和萜类化合物。