The mouse aortic transplantation model is a valuable tool for investigating the mechanisms of atherosclerosis regression, but few laboratories can generate it due to the operation difficulty, especially for the style of end-to-side anastomosis, which facilitates syngeneic heterotopic transplanting a plaque-rich aortic arch into the abdominal aorta. Here we provide a modified protocol for generating this allograft model, which is capable of overcoming several critical surgical challenges such as separating a longer abdominal aorta segment, reducing bleeding and thrombosis, optimizing aortotomy, and improving end-to-side anastomosis to guarantee a potent graft. By transplanting plaque-rich aortic arches into the abdominal aorta of wildtype mice, a high operation success rate (over 90%) was noted with aortic clamping time under 60 min, the graft potency was satisfactory evidenced by examinations of micro-CT, ultrasound, and lower limb blood flow measurement, while a significant atherosclerosis regression was observed in the grafts at 1 week after transplantation.

Graphical Abstract

中文翻译：

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小鼠腹主动脉端侧吻合术同种异体主动脉弓段移植模型

小鼠主动脉移植模型是研究动脉粥样硬化消退机制的重要工具，但由于操作困难，很少有实验室能够制作该模型，尤其是端侧吻合方式，有利于同基因异位移植富含斑块的血管。主动脉弓进入腹主动脉。在这里，我们提供了用于生成该同种异体移植模型的修改方案，该模型能够克服几个关键的手术挑战，例如分离较长的腹主动脉段、减少出血和血栓形成、优化主动脉切开术以及改进端侧吻合以保证有效接枝。将富含斑块的主动脉弓移植到野生型小鼠的腹主动脉中，手术成功率很高（超过90%），主动脉钳夹时间在60分钟以内，显微CT、超声、和下肢血流量测量，移植后 1 周观察到移植物的动脉粥样硬化明显消退。

图形概要