Rind colour is an important quality attribute of melon appearance. Identifying target genes and developing functional molecular markers is significant for rind colour breeding in melon. This study involved a genetic analysis of the fruit rind colours of two inbred lines: H185 (with black green melon rind) and H160 (with white rind) alongside a fruit rind colour gene, CmAPRR2. The purpose was to discover the variation sites of the CmAPRR2 gene and develop specific molecular marker for rind colour in melon. The results showed that the black green rind is dominant over white, and single gene controls the colours. A mutation of the G856T base occurred in the eighth exon of the CmAPRR2 coding DNA region in H160, suggesting that this mutation is the key factor for the white rind colour. Thus, a codominant molecular marker, FC, for gene CmAPRR2 was developed and used for molecular identification of 189 F2 individuals. The marker revealed a complete correspondence between genotype and phenotype. Additionally, the marker revealed that the other four white rinds had G856T mutations. This study provides a basis for targeted improvement of melon rind colours, offering a technical means for molecular marker‐assisted breeding of melon rind colours.

中文翻译：

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瓜皮颜色基因CmAPRR2共显性标记的开发及应用

果皮颜色是甜瓜外观的重要品质属性。识别目标基因并开发功能分子标记对于甜瓜果皮颜色育种具有重要意义。这项研究涉及对两个自交系的果皮颜色进行遗传分析：H185（黑绿色瓜皮）和 H160（白瓜皮）以及果皮颜色基因，CmAPRR2。目的是发现变异位点CmAPRR2基因并开发甜瓜果皮颜色的特异性分子标记。结果表明，黑绿色果皮比白色果皮占优势，单基因控制颜色。G856T碱基突变发生在第8个外显子上CmAPRR2H160 的编码 DNA 区域，表明该突变是白色果皮颜色的关键因素。因此，基因的共显性分子标记 FCCmAPRR2被开发并用于 189 F 的分子鉴定2个人。该标记揭示了基因型和表型之间的完全对应关系。此外，标记显示其他四个白果皮有 G856T 突变。本研究为瓜皮颜色的针对性改良提供了依据，为瓜皮颜色的分子标记辅助育种提供了技术手段。