Arbuscular mycorrhizal fungi and rhizobia are soil symbiotic microorganisms involved in plant nitrogen (N) acquisition, whereas it is unclear how single or combined inoculation of both contributes to soil total N in legume crops. This study analyzed the effects of single or combined inoculation with arbuscular mycorrhizal fungus () and rhizobium ( bv. ) on growth performance, root soluble protein, leghemoglobin, NH-N, NO-N, and N concentrations, nitrogenase activities, soil total N contents, N contents in glomalin-related soil protein (GRSP), as well as the contribution of N in GRSP to soil total N in white clover. Twelve weeks after inoculation, promoted nodule formation, and bv. accelerated root mycorrhizal colonization. Single or combined inoculation with and bv. improved biomass, root total length, surface area and volume, root soluble protein concentrations, nodule leghemoglobin concentrations, and nitrogenase activities to varying degrees, with the trend of improvement being more pronounced with combined inoculation. bv. and/or significantly increased NO-N NH-N, and total N concentrations in nodules and roots, as well as soil total N concentrations. The N in easily extractable GRSP (EE-GRSP) and difficultly extractable GRSP (DE-GRSP) was 4.37–5.64 mg/g and 6.69–7.83 mg/g, respectively, contributing 2.68–3.26 % and 2.28–3.10 % of soil total N. . and combined inoculation accelerated the production of EE-GRSP and DE-GRSP and N in EE-GRSP. . bv. not only significantly increased DE-GRSP production but also reduced N in DE-GRSP. . bv. or significantly increased the contribution of N in DE-GRSP, not EE-GRSP, to soil total N. It was concluded that arbuscular mycorrhizal fungus and rhizobium accelerated N accumulation and contribution to soil total N in white clover by DE-GRSP.

中文翻译：

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丛枝菌根真菌和根瘤菌通过难以提取的球囊霉素相关土壤蛋白促进白三叶植物生长和氮素积累以及对土壤全氮的贡献

丛枝菌根真菌和根瘤菌是参与植物氮（N）获取的土壤共生微生物，但尚不清楚两者的单独或联合接种对豆科作物土壤总氮的影响。本研究分析了丛枝菌根真菌 ( ) 和根瘤菌 ( bv. ) 单独或联合接种对生长性能、根可溶性蛋白、豆血红蛋白、NH-N、NO-N 和 N 浓度、固氮酶活性、土壤全 N 的影响。白三叶草中球囊霉素相关土壤蛋白（GRSP）的含量、球囊霉素相关土壤蛋白（GRSP）中的氮含量以及GRSP中氮对土壤全氮的贡献。接种后十二周，促进结节形成和bv。加速根部菌根定植。单独或联合接种 和 bv。生物量、根总长度、表面积和体积、根可溶性蛋白浓度、根瘤豆血红蛋白浓度、固氮酶活性均有不同程度的提高，其中联合接种提高趋势更为明显。bv. 和/或显着增加NO-N NH-N、根瘤和根中的总氮浓度以及土壤总氮浓度。易提取GRSP（EE-GRSP）和难提取GRSP（DE-GRSP）中的氮含量分别为4.37-5.64 mg/g和6.69-7.83 mg/g，占土壤总量的2.68-3.26%和2.28-3.10%尼.. 联合接种加速了EE-GRSP和DE-GRSP以及EE-GRSP中N的产生。。bv. 不仅显着增加了DE-GRSP产量，而且减少了DE-GRSP中的氮。。bv. 或显着增加了DE-GRSP中的氮对土壤全氮的贡献，而不是EE-GRSP。得出的结论是，丛枝菌根真菌和根瘤菌加速了DE-GRSP中氮的积累和对白三叶草土壤全氮的贡献。