Bryophytes are known bioindicators and are also emerging as effective tools for bioremediation. culture of bryophytes is an important tool for the implementation of several research and industrial applications but it is a poorly explored technology. In this study, we characterize in sterile conditions three aquatic moss species largely used all over the world for decoration but poorly studied: , and . They share interesting morphological traits that suggest their use as natural biofilters. Results include protocols for the establishment of axenic cultures, different for the different species because of their sensitivity to treatments, on which the morphological characters of the three species were described. The sporophytic generation was observed in and but not in that may be unable to develop the diploid generation. The effect of plant growth regulators on gametophyte fragments was described applying 6-benzylaminopurine as cytokinin and α-naphthalene acetic acid as auxin. The absorption of several trace elements was measured in a mixed solution simulating environmental pollution, evidencing specie specificity toward the different elements. The possible applications for these mosses are not only in the field of bioindication but also in bioremediation and environmental restoration. Our study produced widely applicable protocols and basic information for further applications.

中文翻译：

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无菌培养中三种水生苔藓的表征，用于生物监测和生物技术应用

苔藓植物是已知的生物指示剂，也正在成为生物修复的有效工具。苔藓植物培养是实施多项研究和工业应用的重要工具，但它是一项尚未充分探索的技术。在这项研究中，我们在无菌条件下描述了三种水生苔藓物种，它们在世界各地广泛用于装饰，但研究很少： 、 和 。它们具有有趣的形态特征，表明它们可以用作天然生物过滤器。结果包括建立无菌培养物的方案，不同物种因其对处理的敏感性而有所不同，其中描述了三个物种的形态特征。在 和 中观察到孢子体世代，但没有在中观察到可能无法发育二倍体世代。以6-苄氨基嘌呤为细胞分裂素，α-萘乙酸为生长素，描述了植物生长调节剂对配子体片段的影响。在模拟环境污染的混合溶液中测量了几种微量元素的吸收，证明了对不同元素的物种特异性。这些苔藓的可能应用不仅在生物指示领域，而且在生物修复和环境恢复领域。我们的研究产生了广泛适用的方案和进一步应用的基本信息。