Neuroblastoma is a common pediatric malignancy with poor survival for high-risk disease. Mesenchymal stromal cells (MSCs) have innate tumor-homing properties, enabling them to serve as a cellular delivery vehicle, but MSCs have demonstrated variable effects on tumor growth. We compared how placental MSCs (PMSCs) and bone marrow-derived MSCs (BM-MSCs) affect proliferation of neuroblastoma (NB) cells . Indirect co-culture assessed proliferative effects of 18 MSCs (early-gestation PMSCs (n = 9), term PMSCs (n = 5), BM-MSCs (n = 4) on three high-risk NB cell lines (NB1643, SH-SY5Y, and CHLA90). Controls were NB cells cultured in media alone. Proliferation was assessed using MTS assay and measured by fold change (fc) over controls. PMSCs were sub-grouped by neuroprotective effect: strong (n = 7), intermediate (n = 3), and weak (n = 4). The relationship between MSC type, PMSC neuroprotection, and PMSC gestational age on NB cell proliferation was assessed. NB cell proliferation varied between MSC groups. BM-MSCs demonstrated lower proliferative effects than PMSCs (fc 1.18 vs 1.44, p < 0.001). Neither gestational age nor neuroprotection significantly predicted degree of proliferation. Proliferative effects of MSCs varied among NB cell lines. BM-MSCs had less effect on CHLA90 (fc 1.01) compared to NB1643 (fc 1.33) and SH-SY5Y (fc 1.20). Only NB1643 showed a difference between early and term PMSCs (p = 0.04). Effects of MSCs on NB cell proliferation vary by MSC source and NB cell line. BM-MSCs demonstrated lower proliferative effects than most PMSCs. MSC neuroprotection was not correlated with proliferation. Improved understanding of MSC proliferation-promoting mechanisms may provide valuable insight into selection of cells best suited as drug delivery vehicles. N/A. Original Research.

中文翻译：

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间充质基质细胞对神经母细胞瘤细胞系的增殖作用：它们是促进肿瘤还是抑制肿瘤？

神经母细胞瘤是一种常见的儿科恶性肿瘤，高危疾病的生存率较差。间充质基质细胞 (MSC) 具有先天的肿瘤归巢特性，使其能够作为细胞递送载体，但 MSC 已显示出对肿瘤生长的不同影响。我们比较了胎盘间充质干细胞 (PMSC) 和骨髓源性间充质干细胞 (BM-MSC) 如何影响神经母细胞瘤 (NB) 细胞的增殖。间接共培养评估了 18 个 MSC（妊娠早期 PMSC（n = 9）、足月 PMSC（n = 5）、BM-MSC（n = 4）对三种高危 NB 细胞系（NB1643、SH- SY5Y 和 CHLA90）。对照是仅在培养基中培养的 NB 细胞。使用 MTS 测定法评估增殖，并通过相对于对照的倍数变化 (fc) 进行测量。PMSC 根据神经保护作用进行分组：强 (n = 7)、中 (n = 7)、中 (n = 7) n = 3) 和弱 (n = 4)。评估了 MSC 类型、PMSC 神经保护和 PMSC 孕龄对 NB 细胞增殖的关系。不同 MSC 组之间的 NB 细胞增殖情况不同。BM-MSC 的增殖效果低于 PMSC （fc 1.18 vs 1.44，p < 0.001）。胎龄和神经保护均不能显着预测增殖程度。MSC 的增殖效应在 NB 细胞系之间存在差异。与 NB1643 (fc 1.33) 相比，BM-MSC 对 CHLA90 (fc 1.01) 的影响较小) 和 SH-SY5Y (fc 1.20)。只有 NB1643 显示早期和足月 PMSC 之间存在差异 (p = 0.04)。 MSC 对 NB 细胞增殖的影响因 MSC 来源和 NB 细胞系而异。 BM-MSC 表现出比大多数 PMSC 更低的增殖效应。 MSC 的神经保护与增殖不相关。加深对 MSC 增殖促进机制的了解可能会为选择最适合作为药物输送载体的细胞提供有价值的见解。不适用。原创研究。