Efficient detection of cancer-related nucleic acids is pivotal for early cancer diagnosis. This study introduces a target induced three-dimensional DNA biomimetic networks (B-3D Net)-based ratiometric fluorescence platform using manganese dioxide nanosheets (MnO NS)/o-phenylenediamine in combination with hybridization chain reaction to detect cancer-related genes (p53 gene). The incorporation of multiple signals within the B-3D networks can significantly enhance catalytic activity and amplify the output signals, enabling a high sensitivity. Compared with traditional ratio fluorescence platforms, there is no demand to synthesize fluorescent nanoprobes due to the in-situ formation of fluorescence species, which is simple and cost-effective. The corresponding assay demonstrated exceptional sensitivity (with a detection limit as low as 2 fM), selectivity, reproducibility, and accuracy, which mitigates disturbances caused by instrument errors, an inaccurate probe count, and the microenvironment. Furthermore, the ease and straightforwardness of discerning changes in fluorescent brightness and colour by the naked eye are evident. Using the relevant software, a linear relationship between fluorescent images using a smartphone and target concentration was obtained. Hence, the novel ratiometric sensing system will demonstrate new opportunities on determination of target DNA samples in complex biological environments.

中文翻译：

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基于三维DNA仿生网络（B-3D Net）的比率荧光平台用于癌症相关基因生物传感

有效检测癌症相关核酸对于早期癌症诊断至关重要。本研究介绍了基于目标诱导三维DNA仿生网络（B-3D Net）的比例荧光平台，利用二氧化锰纳米片（MnO NS）/邻苯二胺结合杂交链式反应来检测癌症相关基因（p53基因） ）。 B-3D 网络中多种信号的结合可以显着增强催化活性并放大输出信号，从而实现高灵敏度。与传统的比率荧光平台相比，由于荧光物种的原位形成，无需合成荧光纳米探针，简单且成本低廉。相应的检测方法表现出卓越的灵敏度（检测限低至 2 fM）、选择性、重现性和准确性，从而减轻了仪器误差、不准确的探针计数和微环境造成的干扰。此外，通过肉眼辨别荧光亮度和颜色的变化是显而易见的。使用相关软件，获得了使用智能手机的荧光图像与目标浓度之间的线性关系。因此，新型比率传感系统将展示在复杂生物环境中确定目标 DNA 样本的新机会。