Tung tree () as an important industrial raw material used to produce tung oil in China. Genetic transformation system has not been established for the tung tree, hindering research on the functions of tung tree genes and the cultivation of new stress-resistant varieties. In this study, we optimized the tissue culture regeneration system of tung tree hypocotyls, screened out a callus state suitable for infection, and improved the efficiency of genetic transformation. RNA interference (RNAi) vector was constructed using as the target gene. Adventitious shoots were obtained by infecting the hypocotyl calli cultured for 25 days through -mediated infection. The positive adventitious shoot transformation rate of the pBI121 vector was 22.86%, and the adventitious shoot transformation rate of the RNAi vector was 7.84%. We established a complete genetic transformation system using two vectors, and the system was verified via staining and real-time fluorescence quantitative analyses to ensure its stability. This system solves the technical problem of a genetic transformation system for the tung tree, and will provide technical support for studying the functions of tung tree genes and molecular breeding.

中文翻译：

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建立油桐高效愈伤组织遗传转化体系

桐树()作为我国重要的工业原料，用于生产桐油。油桐遗传转化体系尚未建立，阻碍了油桐基因功能研究和抗逆新品种培育。本研究优化了油桐下胚轴组织培养再生体系，筛选出适合感染的愈伤组织状态，提高了遗传转化效率。构建RNA干扰(RNAi)载体作为靶基因。通过介导的感染感染培养25天的下胚轴愈伤组织，获得不定芽。pBI121载体的不定芽阳性转化率为22.86%，RNAi载体的不定芽转化率为7.84%。我们利用两个载体建立了完整的遗传转化体系，并通过染色和实时荧光定量分析验证了该体系，以确保其稳定性。该系统解决了油桐遗传转化体系的技术难题，将为油桐基因功能研究和分子育种提供技术支撑。