This study evaluates compounds isolated from , identifies the chemical composition, and examines its toxicity to mosquito larvae. Liquid chromatography coupled to a mass spectrometer, as well as H and C nuclear magnetic resonance spectroscopy, were used to determine the chemical composition. The 3rd instar and early 4th instar larvae of and were used as test subjects for the activity. There are 4 active ingredients extracted from including 4-hydroxybenzoic acid, pomolic acid, ursolic acid, and daucosterol that have shown low toxicity to (48 h LC of 16.34–22.32 µg/mL), against spp. larvae with 24 h LC values between 28.10 and 55.04 µg/mL; against the larvae of spp. with values of 24 h LC between 8.0 and 17.66 µg/mL, and have shown synergistic or additive effects at binary combinations. This research opens up potential possibilities for developing environmentally friendly natural compounds that kill mosquito larvae and fight dangerous diseases caused by mosquitoes.

中文翻译：

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通过从苎麻中分离出的联合作用化合物控制伊蚊和库蚊幼虫的潜力

这项研究评估了从 中分离出的化合物，鉴定了化学成分，并检查了其对蚊子幼虫的毒性。使用液相色谱与质谱仪以及 H 和 C 核磁共振光谱来确定化学成分。以 和 的 3 龄和 4 龄早期幼虫为实验对象。从其中提取的 4 种活性成分，包括 4-羟基苯甲酸、泊莫酸、熊果酸和胡萝卜甾醇，对 spp 表现出低毒性（48 小时 LC 为 16.34–22.32 µg/mL）。24 小时 LC 值在 28.10 至 55.04 µg/mL 之间的幼虫；针对 spp 的幼虫。24 小时 LC 值在 8.0 至 17.66 µg/mL 之间，并且在二元组合中显示出协同或相加效应。这项研究为开发环境友好的天然化合物开辟了潜在的可能性，这些化合物可以杀死蚊子幼虫并对抗由蚊子引起的危险疾病。