Tyramine is an important index of food freshness degree, and tyrosinase that can specifically oxidized monophenolamine to catecholamine plays a crucial part in the occurrence and development of melanin-related skin diseases. Therefore, it is crucial to develop sensitive and efficient methods for the detection of tyramine and tyrosinase. In this work, encouraged by tyrosinase-triggered specific oxidation of tyramine to dopamine and the unique fluorescent reaction between dopamine and amino silane, we have developed a one-step synthetic strategy of silicon containing nanoparticles (Si CNPs) for “turn-on” detection of tyramine and tyrosinase. The Si CNPs formed with thoroughly studied mechanism exhibit uniform structure and robust yellow-green fluorescence. The low detection limits for tyramine (1.87 μM) and tyrosinase (0.0029 U/mL) demonstrate admirable sensitivity outstripping most methods. The proposed assay achieves satisfactory results in the determination of tyramine and tyrosinase activity in real samples. Furthermore, we leverage this new fluorescent assay to enable the fabrication of an “AND” Boolean logic gate. The entire process can be completed at easily available temperature and pressure with rapid response, convenient operation and visual observation. This fluorescent assay featured with excellent sensitivity, selectivity and stability has considerable prospects in the application of biosensors and disease diagnosis.

中文翻译：

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基于含硅纳米颗粒原位形成的荧光“AND”逻辑门检测酪胺和酪氨酸酶

酪胺是衡量食品新鲜程度的重要指标，而能特异性地将单酚胺氧化为儿茶酚胺的酪氨酸酶在黑色素相关皮肤病的发生、发展中发挥着至关重要的作用。因此，开发灵敏、高效的酪胺和酪氨酸酶检测方法至关重要。在这项工作中，受酪氨酸酶触发的酪胺特异性氧化为多巴胺以及多巴胺和氨基硅烷之间独特的荧光反应的启发，我们开发了一种用于“开启”检测的含硅纳米颗粒（Si CNP）的一步合成策略酪胺和酪氨酸酶。经过深入研究的机制形成的 Si CNP 表现出均匀的结构和强烈的黄绿色荧光。酪胺 (1.87 μM) 和酪氨酸酶 (0.0029 U/mL) 的低检测限证明其灵敏度超过大多数方法。该方法在实际样品中酪胺和酪氨酸酶活性的测定中取得了令人满意的结果。此外，我们利用这种新的荧光测定来制造“AND”布尔逻辑门。整个过程可在容易掌握的温度和压力下完成，反应迅速，操作方便，观察直观。该荧光检测方法具有良好的灵敏度、选择性和稳定性，在生物传感器和疾病诊断中的应用具有广阔的前景。