Mpox is a zoonotic disease caused by mpox virus (MPXV) infection. Since May 2022, there has been a marked increase in human mpox cases in different regions. Rash, fever, and sore throat are typical signs of mpox. However, other viruses, such as the B virus (BV), herpes simplex virus types 1 (HSV-1), herpes simplex virus types 2 (HSV-2), and varicella zoster virus (VZV), can also infect people and cause comparable symptoms. Therefore, clinical symptoms and signs alone make distinguishing MPXV from these viruses difficult. In this study, we combined suspension microarray technology with recombinase-aided amplification technology (RAA) to establish a high-throughput, sensitive, and quantitative method for detecting MPXV and other viruses that can cause similar symptoms. The experimental results confirmed that the technique has outstanding sensitivity, with a minimum detection limit (LOD) of 0.1 fM and a linear range of 0.3 fM to 20 pM, spanning five orders of magnitude. The approach also exhibits exquisite selectivity, as the amplified signal can only be detected when the target virus nucleic acid is present. Additionally, serum recoveries ranging from 80.52% to 119.09% suggest that the detection outcomes are generally considered reliable. Moreover, the time required for detection using this high-throughput method is very short. After DNA extraction, the detection signal amplified by isothermal amplification on the bead array can be obtained in just 1 h. Our research introduces a new technique that utilizes suspension microarray technology and isothermal amplification to create a high-throughput nucleic acid assay. This innovative method offers multiple benefits compared to current techniques, such as being cost-effective, time-efficient, highly sensitive, and having high throughput capabilities. Furthermore, the assay is applicable not only for detecting MPXV and viruses with similar symptoms, but also for clinical diagnostics, food safety, and environmental monitoring, rendering it an effective tool for screening harmful microorganisms.

中文翻译：

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一种基于悬浮微阵列等温扩增的高通量 DNA 分析方法，用于检测 MPOX 病毒和具有类似症状的病毒

Mpox 是由MPXV 感染引起的人畜共患疾病。自2022年5月以来，不同地区的人痘病例明显增加。皮疹、发烧和喉咙痛是痘痘的典型症状。然而，其他病毒，如 B 病毒 (BV)、单纯疱疹病毒 1 型 (HSV-1)、单纯疱疹病毒 2 型 (HSV-2) 和水痘带状疱疹病毒 (VZV) 也可以感染人类并导致类似的症状。因此，仅凭临床症状和体征就很难将 MPXV 与这些病毒区分开来。在本研究中，我们将悬浮微阵列技术与重组酶辅助扩增技术（RAA）相结合，建立了一种高通量、灵敏、定量的方法来检测MPXV和其他可引起类似症状的病毒。实验结果证实，该技术具有出色的灵敏度，最低检测限（LOD）为0.1 fM，线性范围为0.3 fM至20 pM，跨越5个数量级。该方法还表现出精细的选择性，因为只有当目标病毒核酸存在时才能检测到放大的信号。此外，血清回收率范围为 80.52% 至 119.09%，表明检测结果通常被认为是可靠的。而且，使用这种高通量方法检测所需的时间非常短。 DNA提取后，只需1小时即可在微珠阵列上等温扩增得到检测信号。我们的研究引入了一种新技术，利用悬浮微阵列技术和等温扩增来创建高通量核酸测定。与当前技术相比，这种创新方法具有多种优势，例如成本效益高、时间高效、高度灵敏且具有高通量能力。此外，该检测方法不仅适用于检测MPXV和具有类似症状的病毒，还适用于临床诊断、食品安全和环境监测，使其成为筛查有害微生物的有效工具。