Application of wound dressings loaded with antimicrobial agents is considered a viable option to reduce wound bacterial colonization and prevent infection, in order to improve the healing process. In this study, we developed an easy-produced chitosan sponge-like wound dressing containing a broad-host spectrum Escherichia phage BIM BV-67 as an antimicrobial component. This novel dressing was entirely composed of biologically safe and biocompatible materials, had a porous structure, was biodegradable, and exhibited a high swelling capacity. Atomic force microscopy of model layer-by-layer modified planar surfaces and scanning electron microscopy of chitosan sponges showed a non-oriented adsorption and low density of phage particles on chitosan surface. However, immobilized Escherichia phage BIM BV-67 maintained its infectivity and lytic activity. Phage-loaded chitosan sponges effectively inhibited growth of E. coli BIM B-984 G in both qualitative (agar diffusion) and quantitative (suspension) in vitro assays. Moreover, the dressings with phage retained bactericidal activity for 12 weeks when stored at 4 °C. Furthermore, in vivo testing showed that topical application of phage-loaded chitosan sponges significantly reduced the number of E. coli BIM B-984 G cells in the wound and resulted in accelerated wound surface regeneration in infected rats compared to the use of pure sponges and no treatment. Based on these promising results, developed technology has potential as a platform for producing topical dressings with immobilized phages active against wide range of wound-infecting pathogens. In turn, this could promote the application of phage therapy in wound management.

使用含有抗菌剂的伤口敷料被认为是减少伤口细菌定植和预防感染的可行选择，以改善愈合过程。在这项研究中，我们开发了一种易于生产的壳聚糖海绵状伤口敷料，含有广宿主谱大肠杆菌噬菌体 BIM BV-67 作为抗菌成分。这种新型敷料完全由生物安全和生物相容性材料组成，具有多孔结构，可生物降解，并具有高溶胀能力。模型逐层修饰平面的原子力显微镜和壳聚糖海绵的扫描电子显微镜显示壳聚糖表面上噬菌体颗粒的非定向吸附和低密度。然而，固定化的埃希氏菌噬菌体 BIM BV-67 保持了其感染性和裂解活性。在体外定性（琼脂扩散）和定量（悬浮）测定中，负载噬菌体的壳聚糖海绵可有效抑制大肠杆菌BIM B-984 G 的生长。此外，含有噬菌体的敷料在 4°C 保存时可保持 12 周的杀菌活性。此外，体内测试表明，与使用纯海绵相比，局部应用载有噬菌体的壳聚糖海绵可显着减少伤口中大肠杆菌BIM B-984 G 细胞的数量，并加速受感染大鼠的伤口表面再生。没有治疗。基于这些有希望的结果，开发的技术有潜力作为生产外用敷料的平台，该敷料含有固定化噬菌体，可有效对抗多种伤口感染病原体。反过来，这可以促进噬菌体疗法在伤口处理中的应用。