Rapeseed-mustard, the oleiferous Brassica species are important oilseed crops cultivated all over the globe. Mustard aphid Lipaphis erysimi (L.) Kaltenbach is a major threat to the cultivation of rapeseed-mustard. Wild mustard Rorippa indica (L.) Hiern shows tolerance to mustard aphids as a nonhost and hence is an important source for the bioprospecting of potential resistance genes and defense measures to manage mustard aphids sustainably. We performed mRNA sequencing of the R. indica plant uninfested and infested by the mustard aphids, harvested at 24 hours post-infestation. Following quality control, the high-quality reads were subjected to de novo assembly of the transcriptome. As there is no genomic information available for this potential wild plant, the raw reads will be useful for further bioinformatics analysis and the sequence information of the assembled transcripts will be helpful to design primers for the characterization of specific gene sequences. In this study, we also used the generated resource to comprehensively analyse the global profile of differential gene expression in R. indica in response to infestation by mustard aphids. The functional enrichment analysis of the differentially expressed genes reveals a significant immune response and suggests the possibility of chitin-induced defense signaling.

油菜芥菜是含油芸苔属植物，是全球各地种植的重要油料作物。芥菜蚜虫Lipaphis erysimi (L.) Kaltenbach 是油菜芥菜种植的主要威胁。野生芥菜Rorippa indica (L.) Hiern 作为非寄主表现出对芥菜蚜虫的耐受性，因此是潜在抗性基因的生物勘探和可持续管理芥菜蚜虫的防御措施的重要来源。我们对未受芥菜蚜虫侵染和侵染后 24 小时收获的R. indica植物进行了 mRNA 测序。质量控制后，高质量的读数进行转录组的从头组装。由于没有这种潜在野生植物的基因组信息，原始读数将有助于进一步的生物信息学分析，而组装转录本的序列信息将有助于设计用于表征特定基因序列的引物。在本研究中，我们还利用生成的资源全面分析了R. indica应对芥菜蚜虫侵染的差异基因表达的整体概况。差异表达基因的功能富集分析揭示了显着的免疫反应，并表明几丁质诱导的防御信号的可能性。