Fibroblast cycle synchronization in G₀/G₁ is an essential step for nuclear reprogramming by cloning or induced cells to pluripotency. Considering the diversity among rodents and the ecological and scientific importance of these animals, we compared the contact inhibition, serum starvation, and 10 µM of roscovitine as methods of synchronization of red-rumped agouti fibroblasts. The effects of each protocol were evaluated on the percentage of cycle phase, morphology, viability, and apoptosis levels. The results showed that culturing the cells to serum starvation for 24 h (75.9%), 48 h (81.6%), 72 h (86.2%), 96 h (84.0%), and 120 h (83.7%) yielded a significantly higher percentage of cells arrested in the G₀/G₁ (P < 0.05) phase than cells not subjected to any cell cycle synchronization method (31.4%). Also, this effect was not different between the times of 48 and 120 h (P > 0.05). A similar response was observed for cells cultured with roscovitine for 12 h (86.9%), 24 h (74.8%), and 48 h (81.7%), with a higher percentage of synchronized cells in G₀/G₁ compared to cells not submitted to any synchronization treatment (52.2%). Nevertheless, this effect was best evidenced at 12 h (P < 0.05). Also, the contact inhibition for 24–120 h could not synchronize cells in G₀/G₁, with values ranging from 70.9 to 77.9% (P > 0.05). Moreover, no difference was observed for morphology, viability, and apoptosis levels in any synchronization method (P > 0.05). Therefore, serum starvation is as efficient as roscovitine on cycle synchronization in G₀/G₁ of red-rumped agouti fibroblasts.

_{G 0} /G ₁中的成纤维细胞周期同步是通过克隆或诱导细胞多能性进行核重编程的重要步骤。考虑到啮齿类动物的多样性以及这些动物的生态和科学重要性，我们比较了接触抑制、血清饥饿和 10 µM roscovitine 作为红腰刺豚鼠成纤维细胞同步化的方法。评估每个方案对周期阶段百分比、形态、活力和凋亡水平的影响。结果显示，将细胞培养至血清饥饿24小时（75.9％）、48小时（81.6％）、72小时（86.2％）、96小时（84.0％）和120小时（83.7％）时，产量显着更高 与未进行任何细胞周期同步方法的细胞相比，停滞在 G ₀ /G ₁ ( P < 0.05) 期的细胞百分比 (31.4%)。此外，这种效果在 48 小时和 120 小时之间没有差异（P  > 0.05）。用 roscovitine 培养 12 小时（86.9%）、24 小时（74.8%）和 48 小时（81.7%）的细胞也观察到类似的反应，与未培养的细胞相比，G ₀ /G ₁同步细胞的百分比更高接受任何同步治疗（52.2%）。然而，这种效果在 12 小时时得到了最好的证明（P  < 0.05）。此外，24-120 h的接触抑制不能使G ₀ /G ₁中的细胞同步，其值范围为70.9%至77.9%（P  > 0.05）。此外，在任何同步方法中，形态、活力和凋亡水平均未观察到差异（P  > 0.05）。因此，血清饥饿对于红腰刺鼠成纤维细胞G ₀ /G ₁的周期同步化与roscovitine 一样有效。