Abiotic stressors like waterlogging are detrimental to cucumber development and growth. However, comprehension of the highly complex molecular mechanism underlying waterlogging can provide an opportunity to enhance cucumber tolerance under waterlogging stress. We examined the hypocotyl and stage-specific transcriptomes of the waterlogging-tolerant YZ026A and the waterlogging-sensitive YZ106A, which had different adventitious rooting ability under waterlogging. YZ026A performed better under waterlogging stress by altering its antioxidative machinery and demonstrated a greater superoxide ion (O2-) scavenging ability. $$CsPrx73$$CsPrx73$$under waterlogging condition $$ Silencing of CsPrx73 expression by VIGS adversely affects AR formation under waterlogging condition. Our results also indicated that CsERF7-3, a waterlogging-responsive ERF transcription factor, can directly bind to the ATCTA-box motif in the CsPrx73 promoter to initiate its expression. Overexpression of CsERF7-3 enhanced CsPrx73 expression and $$. On the contrary, CsERF7-3 -silenced plants decreased CsPrx73 expression and rooting ability. Ultimately, our study demonstrates a novel CsERF7-3-CsPrx73 module that allows cucumbers to adapt more efficiently to waterlogging stress by promoting AR production and ROS scavenging.

中文翻译：

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基于RNA-seq的比较转录组分析揭示了CsPrx73在淹水触发黄瓜不定根形成中的作用

涝渍等非生物应激因素不利于黄瓜的发育和生长。然而，理解涝害背后高度复杂的分子机制可以为增强黄瓜在涝害胁迫下的耐受性提供机会。我们检查了耐涝YZ026A和涝敏感YZ106A的下胚轴和阶段特异性转录组，它们在涝渍下具有不同的不定根能力。YZ026A 通过改变其抗氧化机制，在涝害胁迫下表现更好，并表现出更强的超氧离子 (O2-) 清除能力。$$CsPrx73$$CsPrx73$$在涝灾条件下$$ VIGS 沉默 CsPrx73 表达会对涝灾条件下 AR 的形成产生不利影响。我们的结果还表明，CsERF7-3（一种淹水响应性ERF转录因子）可以直接与CsPrx73启动子中的ATCTA-box基序结合以启动其表达。CsERF7-3 的过表达增强了 CsPrx73 的表达和 $$。相反，CsERF7-3沉默的植物降低了CsPrx73表达和生根能力。最终，我们的研究展示了一种新颖的 CsERF7-3-CsPrx73 模块，该模块可以通过促进 AR 产生和 ROS 清除来使黄瓜更有效地适应涝害压力。