As a vital component of blood, platelets play crucial roles in hemostasis and maintaining vascular integrity, and actively participate in inflammation and immune regulation. The unique biological properties of natural platelets have enabled their utilization as drug delivery vehicles. The advancement and integration of various techniques, including biological, chemical, and physicochemical methods, have enabled the preparation of engineered platelets. Platelets can serve as drug delivery platforms combined with immunotherapy and chemokine therapy to enhance their therapeutic impact. This review focuses on the recent advancements in the application of unactivated platelets for drug delivery. The construction strategies of engineered platelets are comprehensively summarized, encompassing internal loading, surface modification, and genetic engineering techniques. Engineered platelets hold vast potential for treating cardiovascular diseases, cancers, and infectious diseases. Furthermore, the challenges and potential considerations in creating engineered platelets with natural activity are discussed.

中文翻译：

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工程未活化血小板用于靶向药物输送

血小板作为血液的重要组成部分，在止血和维持血管完整性方面发挥着至关重要的作用，并积极参与炎症和免疫调节。天然血小板独特的生物学特性使其能够用作药物输送载体。包括生物、化学和物理化学方法在内的各种技术的进步和整合使得工程血小板的制备成为可能。血小板可以作为药物输送平台，与免疫疗法和趋化因子疗法相结合，以增强其治疗效果。本综述重点关注未活化血小板在药物输送方面的应用的最新进展。全面总结了工程血小板的构建策略，包括内部负载、表面修饰和基因工程技术。工程血小板在治疗心血管疾病、癌症和传染病方面具有巨大潜力。此外，还讨论了制造具有天然活性的工程血小板的挑战和潜在考虑因素。