Domestic dogs have great potential to expand our understanding of the determinants of aging. To understand the aging pattern of domestic dogs and evaluate whether they can be used as an aging model, we performed RNA sequencing on white blood cells from domestic dogs aged 1–9 years and treated aged dogs with classical antiaging approaches. We obtained 30 RNA sequencing libraries and identified 61 age‐associated genes with dynamic changes, the majority of which were related to metabolism and immune function, which may be predominant biomarkers for aging in dogs. We next treated aged dogs with canine mesenchymal stem cells (cMSCs), nicotinamide mononucleotide, and rapamycin to determine whether and how they responded to the antiaging interventions. The results showed that these treatments can significantly reduce the level of inflammatory factors (IL‐6 and TNF‐α). MSCs effectively improved the heart functions of aged dogs. Three key potential age‐related genes (PYCR1, CCRL2, and TOX) were reversed by MSC treatment, two of which (CCRL2 and TOX) are implicated in immunity. Overall, we profiled the transcriptomic pattern of domestic dogs and revealed that they may be a good model of aging, especially in anti‐inflammatory investigations.

中文翻译：

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转录组学和干预证据表明家犬是抗炎研究的有前途的模型

家养狗在扩大我们对衰老决定因素的理解方面具有巨大的潜力。为了了解家犬的衰老模式并评估它们是否可以用作衰老模型，我们对1-9岁家犬的白细胞进行了RNA测序，并用经典的抗衰老方法对老年犬进行了治疗。我们获得了30个RNA测序文库，鉴定了61个具有动态变化的年龄相关基因，其中大多数与代谢和免疫功能相关，这些基因可能是狗衰老的主要生物标志物。接下来，我们用犬间充质干细胞 (cMSC)、烟酰胺单核苷酸和雷帕霉素治疗老年狗，以确定它们是否以及如何对抗衰老干预措施做出反应。结果表明，这些治疗可以显着降低炎症因子（IL-6和TNF-α）的水平。间充质干细胞有效改善老年犬的心脏功能。三个关键的潜在年龄相关基因（PYCR1、CCRL2， 和毒性）通过 MSC 治疗得到逆转，其中两个（CCRL2和毒性）与免疫力有关。总的来说，我们分析了家养狗的转录组模式，并揭示它们可能是一个很好的衰老模型，特别是在抗炎研究中。