Abstract

To understand the lifespan of higher organisms, including humans, it is important to understand lifespan at the cellular level as a prerequisite. So, fission yeast is a good model organism for the study of lifespan. To identify the novel factors involved in longevity, we are conducting a large-scale screening of long-lived mutant strains that extend chronological lifespan (cell survival in the stationary phase) using fission yeast. One of the newly acquired long-lived mutant strains (No.98 mutant) was selected for analysis and found that the long-lived phenotype was due to a missense mutation (92Phe → Ile) in the plb1⁺ gene. plb1⁺ gene in fission yeast is a nonessential gene encoding a homolog of phospholipase B, but its functions under normal growth conditions, as well as phospholipase B activity, remain unresolved. Our analysis of the No.98 mutant revealed that the plb1 mutation reduces the integrity of the cellular membrane and cell wall and activates Sty1 via phosphorylation.

中文翻译：

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通过激活粟酒裂殖酵母中的 Sty1 MAPK 延长寿命的 plb1 突变的鉴定

摘要

要了解包括人类在内的高等生物的寿命，首先必须了解细胞水平的寿命。因此，裂殖酵母是研究寿命的良好模式生物。为了确定与长寿相关的新因素，我们正在使用裂殖酵母对长寿突变菌株进行大规模筛选，这些突变菌株可延长时间寿命（稳定期的细胞存活率）。选择一株新获得的长寿突变株（No.98突变体）进行分析，发现其长寿表型是由于plb1 ⁺基因中的错义突变（92Phe→Ile）所致。裂殖酵母中的plb1 ⁺基因是编码磷脂酶 B 同源物的非必需基因，但其在正常生长条件下的功能以及磷脂酶 B 活性仍未解决。我们对 No.98 突变体的分析表明，plb1突变降低了细胞膜和细胞壁的完整性，并通过磷酸化激活 Sty1。