Myocardial infarction (MI) results in an impaired heart function. Conductive hydrogel patch-based therapy has been considered as a promising strategy for cardiac repair after MI. In our study, we fabricated a three-dimensional (3D) printed conductive hydrogel patch made of fibrinogen scaffolds and mesenchymal stem cells (MSCs) combined with graphene oxide (GO) flakes (MSC@GO), capitalizing on GO’s excellent mechanical property and electrical conductivity. The MSC@GO hydrogel patch can be attached to the epicardium via adhesion to provide strong electrical integration with infarcted hearts, as well as mechanical and regeneration support for the infarcted area, thereby up-regulating the expression of connexin 43 (Cx43) and resulting in effective MI repair in vivo. In addition, MI also triggers apoptosis and damage of cardiomyocytes (CMs), hindering the normal repair of the infarcted heart. GO flakes exhibit a protective effect against the apoptosis of implanted MSCs. In the mouse model of MI, MSC@GO hydrogel patch implantation supported cardiac repair by reducing cell apoptosis, promoting gap connexin protein Cx43 expression, and then boosting cardiac function. Together, this study demonstrated that the conductive hydrogel patch has versatile conductivity and mechanical support function and could therefore be a promising candidate for heart repair.

中文翻译：

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3D 打印导电水凝胶贴片与 MSC@GO 结合，可有效修复心肌梗死

心肌梗塞 (MI) 会导致心脏功能受损。基于导电水凝胶贴片的治疗被认为是心肌梗死后心脏修复的一种有前途的策略。在我们的研究中，我们利用GO优异的机械性能和电学性能，制作了由纤维蛋白原支架和间充质干细胞（MSC）与氧化石墨烯（GO）薄片（MSC@GO）相结合制成的三维（3D）打印导电水凝胶贴片。电导率。 MSC@GO水凝胶贴片可以通过粘附作用附着在心外膜上，为梗塞心脏提供强大的电整合，并为梗塞区域提供机械和再生支持，从而上调连接蛋白43（Cx43）的表达，从而产生有效修复体内MI 。此外，心肌梗死还会引发心肌细胞（CM）凋亡和损伤，阻碍梗塞心脏的正常修复。 GO 薄片对植入的 MSC 细胞凋亡具有保护作用。在 MI 小鼠模型中，MSC@GO 水凝胶贴片植入通过减少细胞凋亡、促进间隙连接蛋白 Cx43 表达、进而增强心脏功能来支持心脏修复。总之，这项研究表明，导电水凝胶贴片具有多功能的导电性和机械支撑功能，因此可能成为心脏修复的有希望的候选者。