Trichinella spiralis infection is a food‐borne zoonotic disease caused by nematodes that dwell in the tissues, presenting a significant public health concern. This study aimed to evaluate the effectiveness of different treatments including silver nanoparticles (AgNPs), myrrh biosynthesized AgNPs “AgNPs synthesized using plant‐based green technologies”, myrrh extract, and myrrh essential oil, as alternative treatments against T. spiralis infection. Parasitological, histopathological, and cytotoxicity assessments were conducted to investigate the effects of various concentrations of these treatments in reducing the populations of adult worms and larvae during both the intestinal and muscular phases of T. spiralis‐infected mice. The results showed that the highest antihelminthic efficacy against the intestinal phase of T. spiralis was achieved by myrrh extract (86.66%), followed closely by AgNPs (84.96%) and myrrh AgNPs (82.51%) at higher concentrations (800 mg/kg for myrrh extract, 40 μg/mL for AgNPs, and 40 μg/mL for myrrh AgNPs). While the group treated with myrrh essential oil showed the lowest percentage of adult reduction (78.14%). However, all treatments demonstrated comparable effects in reducing the larvae population in the muscle phase. Histopathological examination of the tissues revealed compelling evidence of the effectiveness of AgNPs, particularly when prepared with myrrh. Additionally, a comprehensive assessment of the cytotoxicity of AgNPs indicated low toxicity levels. This study supports that AgNPs synthesized using plant‐based green technologies hold therapeutic potential for the treatment of T. spiralis infection. These findings present a promising avenue for the development of novel antiparasitic drugs that are both effective and safe.Research Highlights Myrrh extract has the highest antihelminthic efficacy against the intestinal phase of T. spiralis. Histopathological examination of the tissues revealed compelling evidence of the effectiveness of AgNPs, particularly when prepared with myrrh. During intestinal phase of T. spiralis, varying levels of nanoparticle precipitation were detected in the liver, brain, lung, and intestine. During the muscular phase, the highest amount of AgNPs precipitation was detected in the liver, followed by the brain, and lung.

中文翻译：

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光学显微镜和寄生虫学分析揭示了银纳米颗粒和各种没药提取物对小鼠旋毛虫感染的有益作用

旋毛虫感染是一种由寄居在组织中的线虫引起的食源性人畜共患疾病，引起了重大的公共卫生问题。本研究旨在评估不同治疗方法的有效性，包括银纳米粒子（AgNP）、没药生物合成 AgNP（“使用植物绿色技术合成的 AgNP”）、没药提取物和没药精油作为替代疗法旋毛虫感染。进行了寄生虫学、组织病理学和细胞毒性评估，以研究不同浓度的这些处理对减少肠道和肌肉阶段成虫和幼虫数量的影响。T.spiralis-被感染的老鼠。结果表明，对肠期的驱虫效果最高。旋毛虫没药提取物 (86.66%) 实现了这一目标，紧随其后的是较高浓度的 AgNPs (84.96%) 和没药 AgNPs (82.51%)（没药提取物为 800 mg/kg，AgNPs 为 40 μg/mL，AgNPs 为 40 μg/mL）。没药 AgNP)。而没药精油治疗组的成人减少百分比最低（78.14%）。然而，所有治疗方法在减少肌肉期幼虫数量方面都表现出类似的效果。对组织的组织病理学检查揭示了 AgNP 有效性的令人信服的证据，特别是在用没药制备时。此外，对 AgNP 细胞毒性的综合评估表明其毒性水平较低。这项研究表明，使用基于植物的绿色技术合成的 AgNP 具有治疗以下疾病的潜力：旋毛虫感染。这些发现为开发既有效又安全的新型抗寄生虫药物提供了一条有希望的途径。 研究亮点 没药提取物对肠道阶段的驱虫功效最高旋毛虫。 对组织的组织病理学检查揭示了 AgNP 有效性的令人信服的证据，特别是在用没药制备时。 在肠期旋毛虫在肝脏、大脑、肺和肠道中检测到不同水平的纳米颗粒沉淀。在肌肉阶段，在肝脏中检测到最高量的纳米颗粒沉淀，其次是大脑和肺。