This experiment evaluated the effects of an analogue of bovine appeasing substance (BAS) on growth, stress, handling quality, and reproduction of Nellore heifers. Twenty-nine heifers [body condition score (BCS) = 4.84 ± 0.13; body weight (BW) = 371 ± 0.77 kg; 23 ± 2 months of age)] were assigned to 1 of 2 treatments: 1) Saline solution (0.9 NaCl; n = 14) or BAS (Secure Cattle, IRSEA Group, Quartier Salignan, France; n = 15). Both solutions were applied topically in the nuchal skin area (5 mL/heifers) at the beginning (d -9) of the fixed-time artificial insemination protocol (FTAI). Heifers were allocated in two different pastures according to the treatment (one pasture per treatment) for 15 d (time of action of BAS) and then combined in a single group (both treatments in the same pasture). Heifers were inseminated on d 0, and BW and BCS were collected on d -9 and 40. The temperament in the squeeze chute (entry and exit scores; scale 1 – 3; chute score; scale 1 – 5; greater values mean more reactive heifers) and blood samples were collected on d -9, 0, 7, 14 and 30. The percentual of aggression, accident, and dirtiness in the perineal region were evaluated on d 0 along with time to inseminate, estrus expression scores (scale 1 – 3; 1 = no expression, 2 = low expression, 3 = greater expression), and dominant follicle size. The corpus luteum size was measured on d 7 and 14, and the pregnancy and embryo size on d 40. The BAS vs. Saline-treated heifers tended to have greater BW change (23.6 vs. 20.8 ± 1.03 kg; P = 0.09) and had greater BCS change (0.05 vs. -0.21 ± 0.05; P < 0.01). The BAS vs. Saline-treated heifers tended to have lesser entry scores (1.42 vs. 1.67 ± 0.09; P = 0.09) and percentual of dirtiness in the perineal region (48.3 vs. 71.1 ± 8.67 %; P = 0.06), and no effects of treatment were detected (P ≥ 0.39) for chute score, exit score, aggression, accident and time to inseminate. The BAS vs. Saline-treated heifers had greater estrus scores (2.00 vs. 1.27 ± 0.21; P = 0.03), and no effects were detected (P ≥ 0.11) for ovarian variables, pregnancy rate, and conceptus size. Furthermore, no treatment effects were detected (P ≥ 0.25) for serum concentration of cortisol and plasma concentration of haptoglobin, ceruloplasmin, and progesterone. Therefore, BAS application at the beginning of the FTAI protocol could decrease the temperament and dirtiness in the perineal region and increase the growth and estrus expression of beef heifers.

中文翻译：

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130 与固定时间 AI 同步的安抚物质应用对 Bos indicus 小母牛生长、应激、处理质量和繁殖的影响

该实验评估了牛安抚物质 (BAS) 类似物对内洛尔小母牛的生长、应激、处理质量和繁殖的影响。二十九头小母牛[身体状况评分（BCS）= 4.84 ± 0.13；体重（BW）=371±0.77公斤；23 ± 2 月龄）] 被分配到 2 种治疗中的 1 种：1）盐水溶液（0.9 NaCl；n = 14）或 BAS（Secure Cattle，IRSEA Group，Quartier Salignan，法国；n = 15）。在固定时间人工授精方案 (FTAI) 开始 (d -9) 时，将两种溶液局部应用于颈部皮肤区域 (5 mL/小母牛)。根据处理情况，将小母牛分配到两个不同的牧场（每个处理一个牧场），持续 15 天（BAS 作用时间），然后合并为一个组（两种处理都在同一牧场）。小母牛在第 0 天进行授精，并在第 -9 天和第 40 天收集 BW 和 BCS。挤压槽中的性情（进入和退出分数；等级 1 – 3；槽分数；等级 1 – 5；值越大意味着反应性越高）在第 -9、0、7、14 和 30 天收集血液样本。在第 0 天评估会阴区域的攻击性、事故和肮脏的百分比以及授精时间、发情表达评分（量表 1） – 3；1 = 无表达，2 = 低表达，3 = 较高表达）和优势卵泡大小。在第 7 天和第 14 天测量黄体大小，在第 40 天测量妊娠和胚胎大小。BAS 与盐水处理的小母牛相比，体重变化往往更大（23.6 与 20.8 ± 1.03 kg；P = 0.09），并且具有更大的 BCS 变化（0.05 对比 -0.21 ± 0.05；P < 0.01）。BAS 与生理盐水处理的小母牛相比，入口得分（1.42 vs. 1.67 ± 0.09；P = 0.09）和会阴区域脏污百分比（48.3 vs. 71.1 ± 8.67 %；P = 0.06）往往较低，并且没有检测治疗效果（P ≥ 0.39）：滑道评分、退出评分、攻击性、事故和授精时间。BAS 与生理盐水处理的小母牛相比，发情评分更高（2.00 vs. 1.27 ± 0.21；P = 0.03），并且未检测到对卵巢变量、妊娠率和概念大小的影响 (P ≥ 0.11)。此外，未检测到皮质醇血清浓度和触珠蛋白、血浆铜蓝蛋白和黄体酮浓度的治疗效果（P≥0.25）。因此，在 FTAI 方案开始时应用 BAS 可以降低会阴区域的气质和肮脏程度，并增加肉用小母牛的生长和发情表现。