The importance of embryo biopsy is running a 50K genomic test will be completed on the DNA biopsy, giving us the ability to collect genetic prediction data before the calf is even born. The objective of this study was to compare the viability of bovine embryos (n = 24) at different stages of embryo maturation, after microblade biopsy and embryo transfer. This process was achieved by taking a small biopsy of each embryo. The results of aspirating oocytes from packing house ovaries and in vitro fertilization (IVF), within a 48 h time period marked d 0. After a 7-d incubation period, the results consisted of 14 blastocysts and 10 early blastocysts. The final sample size, of 24 embryos, differing in stages between early blastocyst (TRT 6) and blastocyst (TRT 7). All the embryos, except for one, was a Grade 1 embryo. On d 7 the embryos were grouped by stage of embryo. Each embryo was washed, and biopsied with a microblade by a single technician. After the biopsy, the embryos were incubated for a 24 h period. Immediately after the incubation period, each embryo was analyzed for viability. On d 8, TRT 7 embryos had a 50% viability rate (n = 7), whereas TRT 6 embryos had a 70% viability rate (n = 7). Data were analyzed using GLIMMIX procedure of SAS 9.4. The viability did not differ between either treatment (P = 0.58). However, the biopsy of each embryo resulted in a significant effect on embryo viability (P < 0.01). In conclusion, using embryo biopsy and the stage of the embryo may affect embryo viability.

中文翻译：

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96 胚胎阶段和活检对胚胎活力的影响

胚胎活检的重要性在于对 DNA 活检进行 50K 基因组测试，使我们能够在小牛出生之前收集遗传预测数据。本研究的目的是比较微刀片活检和胚胎移植后牛胚胎 (n = 24) 在胚胎成熟不同阶段的活力。这个过程是通过对每个胚胎进行小活检来实现的。48 小时内从包装厂卵巢抽吸卵母细胞和体外受精 (IVF) 的结果标记为 d 0。经过 7 天的孵化期后，结果包括 14 个囊胚和 10 个早期囊胚。最终样本大小为 24 个胚胎，早期囊胚 (TRT 6) 和囊胚 (TRT 7) 之间的阶段不同。除一颗胚胎外，所有胚胎均为一级胚胎。第 7 天，按胚胎阶段对胚胎进行分组。每个胚胎均由一名技术人员清洗并用微刀片进行活检。活检后，将胚胎培育24小时。孵化期结束后，立即分析每个胚胎的活力。第 8 天，TRT 7 胚胎的存活率为 50%（n = 7），而 TRT 6 胚胎的存活率为 70%（n = 7）。使用 SAS 9.4 的 GLIMMIX 程序分析数据。两种处理之间的活力没有差异（P = 0.58）。然而，每个胚胎的活检对胚胎活力产生显着影响(P＜0.01)。总之，使用胚胎活检和胚胎的阶段可能会影响胚胎的活力。