The extracellular matrix (ECM) plays a crucial role in regulating cellular behaviors and functions. However, the impact of ECM topography on muscle cell adhesion and differentiation remains poorly understood from a mechanosensing perspective. In this study, we fabricated aligned and random electrospun polycaprolactone (PCL) nanofibers to mimic the structural characteristics of ECM. Mechanism investigations revealed that the orientation of nanofibers promoted C2C12 polarization and myogenesis through Rac-related signaling pathways. Conversely, cells cultured on random fibers exhibited spreading behavior mediated by RhoA/ROCK pathways, resulting in enhanced stress fiber formation but reduced capacity for myogenic differentiation. Our findings highlight the critical role of an ECM structure in muscle regeneration and damage repair, providing novel insights into mechanosensing mechanisms underlying muscle injury diseases.

中文翻译：

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对齐纳米纤维通过激活 Rac 相关信号通路促进成肌细胞极化和肌生成

细胞外基质（ECM）在调节细胞行为和功能中发挥着至关重要的作用。然而，从机械传感的角度来看，ECM 地形对肌肉细胞粘附和分化的影响仍然知之甚少。在这项研究中，我们制造了定向和随机静电纺丝聚己内酯（PCL）纳米纤维来模拟 ECM 的结构特征。机制研究表明，纳米纤维的方向通过 Rac 相关信号通路促进 C2C12 极化和肌生成。相反，在随机纤维上培养的细胞表现出由 RhoA/ROCK 途径介导的铺展行为，导致应力纤维形成增强，但生肌分化能力降低。我们的研究结果强调了 ECM 结构在肌肉再生和损伤修复中的关键作用，为肌肉损伤疾病背后的机械传感机制提供了新的见解。