Artificial dermal scaffolds (ADSs) have great value in repairing deep skin defects. However, problems such as unsatisfactory angiogenesis and local dropsy or empyema often occur, resulting in delayed or even failed wound healing. Negative pressure wound therapy (NPWT) is an effective therapy to promote wound healing or shorten wound bed preparation time. Studies on whether it can improve the effects of ADSs have never been interrupted, and no consensus has been reached. In this study, an improved ADS was prepared by mesh technology, physicochemical experiments were conducted, cell adhesion and proliferation were assessed with the meshed ADS, and in vivo experiments were conducted to investigate the effects of meshed ADS or ADS combined with NPWT in repairing full-thickness skin defects. The results showed that the meshed ADS showed through-layer channels arranged in parallel longitudinal and transverse intersections. The cell experiments confirmed the good cytocompatibility. The in vivo experiments showed that there were no differences in the take rate or contraction of grafted skin among all experiment groups. The meshed ADS exhibited good histocompatibility, and there were no differences in tissue inflammation, dermal angiogenesis, or degradation among all groups. In addition, necrosis, dropsy, or empyema of the dermal scaffold were found in all experiment groups except for the meshed ADS + NPWT group, which showed better wound repair results, including fewer scaffold-related complications and satisfactory skin graft survival and wound contraction. In conclusion, this novel meshed ADS, which has a regular through-layer mesh structure and possesses stable physicochemical properties and good biocompatibility, combined with NPWT can ensure adequate subdermal drainage and reduce the risk of scaffold-related complications, thereby improving the quality and efficiency of wound repair, promoting a broader application of biomaterials, and helping physicians and readers implement more effective wound management.

中文翻译：

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网状人工真皮支架和负压伤口疗法在治疗全层皮肤缺损中的应用：一项前瞻性体内研究

人工真皮支架（ADS）在修复深层皮肤缺损方面具有巨大价值。但常常会出现血管生成不理想、局部水肿或脓胸等问题，导致伤口愈合延迟甚至失败。负压伤口治疗（NPWT）是促进伤口愈合或缩短创面床准备时间的有效疗法。关于它是否能够改善ADS效果的研究一直没有间断，也没有达成共识。本研究采用网状技术制备了改进的ADS，进行理化实验，用网状ADS评估细胞粘附和增殖，并进行体内实验研究网状ADS或ADS联合NPWT修复全组织的效果。 - 厚皮缺陷。结果表明，网状 ADS 显示出平行纵向和横向交叉排列的穿层通道。细胞实验证实了良好的细胞相容性。体内实验表明，各实验组移植皮的取出率和收缩率均无差异。网状ADS表现出良好的组织相容性，各组之间的组织炎症、真皮血管生成或降解没有差异。此外，除网状ADS+NPWT组外，所有实验组均发现真皮支架坏死、水肿或脓胸，其伤口修复效果较好，包括支架相关并发症较少，植皮成活和伤口收缩令人满意。综上所述，这种新型网状ADS具有规则的穿层网状结构，具有稳定的理化性质和良好的生物相容性，与NPWT结合可以保证皮下充分引流，降低支架相关并发症的风险，从而提高质量和效率伤口修复，促进生物材料更广泛的应用，并帮助医生和读者实施更有效的伤口管理。