Gomphonema parvulum is a cosmopolitan freshwater diatom that is used as an indicator in water quality biomonitoring. In this study, we report the culturing of two geographically separated isolates from southeastern North America, their morphology, and the sequencing and assembly of their mitochondrial and chloroplast genomes. Morphologically, both strains fit G. parvulum sensu lato, but the frustules from a protected habitat in South Carolina were smaller than those cited in the historic data of this species from the same location as well as a second culture from Virginia. Phylogenetic analyses using the rbcL gene placed both within a clade with G. parvulum. Genetic markers, including full chloroplast and mitochondrial genomes and the nuclear small subunit rRNA gene region were assembled from each isolate. The organellar genomes of the two strains varied slightly in size due to small differences in intergenic regions with chloroplast genomes of 121,035 bp and 121,482 bp and mitochondrial genomes of 34,639 bp and 34,654 bp. The intraspecific pairwise identities of the chloroplast and mitochondrial genomes of these two isolates were 97.9% and 95.4%, respectively. Multigene phylogenetic analysis demonstrated a close relationship between G. parvulum, Gomphoneis minuta, and Didymosphenia geminata.

中文翻译：

---

来自南卡罗来纳州（美国）和弗吉尼亚州（美国）的两种 Gomphonema parvulum（硅藻门）环境分离株的叶绿体和线粒体基因组

Gomphonema parvulum是一种世界性的淡水硅藻，用作水质生物监测的指示剂。在这项研究中，我们报告了来自北美东南部的两个地理上分离的分离株的培养、它们的形态、以及它们的线粒体和叶绿体基因组的测序和组装。从形态上看，两种菌株均符合G。  parvulum sensu lato，但来自南卡罗来纳州受保护栖息地的硅藻壳比来自同一地点的该物种以及来自弗吉尼亚州的第二种文化的历史数据中引用的那些要小。使用rbc L 基因进行系统发育分析，将两者置于具有G 的进化枝内。 小儿。从每个分离物中组装了遗传标记，包括完整的叶绿体和线粒体基因组以及核小亚基 rRNA 基因区域。由于基因间区域差异较小，两个菌株的细胞器基因组大小略有不同，叶绿体基因组为121,035 bp和121,482 bp，线粒体基因组为34,639 bp和34,654 bp。这两个分离株的叶绿体和线粒体基因组的种内配对同一性分别为97.9%和95.4%。多基因系统发育分析证明了G之间的密切关系。  parvulum、Gomphoneis minuta和Didymosphenia geminata。