Liriope spicata is an important traditional Chinese medicine and is also used as an ornamental groundcover plant. A tissue culture method, such as somatic embryo regeneration, that can generate large numbers of genetically identical plants would help maintain the characteristics of specific varieties. This study aimed to screen and optimize plant growth regulators for all aspects of somatic embryo regeneration in L. spicata. The plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (6-BA), naphthalene acetic acid (NAA), gibberellic acid (GA₃), indole-3-acetic acid (IAA), and kinetin (KT) were screened in tissue culture media. We found that 2 mg/L 2,4-D + 2 mg/L 6-BA + 0.1 mg/L NAA had the best induction efficiency of 30.95%. During the screening of the somatic embryo proliferation media, we found that MS + 1 mg/L 6-BA + 0.1 mg/L NAA + 20 g/L glucose + 7 g/L agar, with a pH of 5.8, had the best proliferation efficiency of 86.67%. For the germination of somatic embryos, we found that MS + 0.1 mg/L 6-BA + 0.2 mg/L NAA + 1 mg/L GA₃ had the best effect, with an efficiency of 29.03%. Indole-3-butyric acid (IBA) had a significant effect on the induction of adventitious shoots, with the formula 1/2 MS + 0.1 mg/L IBA resulting in an efficiency of 100%. The establishment of the regeneration pathway for L. spicata somatic embryos not only ensures the industrial production of species but also provides reference for the application of plant growth regulators in plant tissue culture.

麦冬是重要的中药材，也用作观赏地被植物。组织培养方法，例如体细胞胚胎再生，可以产生大量基因相同的植物，这将有助于保持特定品种的特征。本研究旨在筛选和优化用于L. spicata体细胞胚胎再生各个方面的植物生长调节剂。植物生长调节剂2,4-二氯苯氧乙酸(2,4-D)、6-苄氨基嘌呤(6-BA)、萘乙酸(NAA)、赤霉酸(GA ₃ )、吲哚-3-乙酸(IAA)和激动素（KT）在组织培养基中进行筛选。我们发现2 mg/L 2,4-D + 2 mg/L 6-BA + 0.1 mg/L NAA 的诱导效率最好，为30.95%。在体细胞胚增殖培养基的筛选中，我们发现MS+1 mg/L 6-BA+0.1 mg/L NAA+20 g/L葡萄糖+7 g/L琼脂，pH为5.8，效果最好。增殖效率达86.67%。对于体细胞胚的萌发，我们发现MS+0.1 mg/L 6-BA+0.2 mg/L NAA+1 mg/L GA ₃效果最好，效率为29.03%。3-吲哚丁酸(IBA)对不定芽的诱导有显着效果，配方为1/2 MS+0.1 mg/L IBA，效率达100%。留香体细胞胚再生途径的建立，不仅保证了物种的工业化生产，也为植物生长调节剂在植物组织培养中的应用提供参考。