We have functionally evaluated a transcription factor for its role in pepper immune responses to attack and high temperature-high humidity (HTHH). Exposure to . inoculation (RSI) and HTHH resulted in up-regulation of this nucleus-localized TF. Function of this TF was confirmed by performing loss of function assay of CaMYB59 by VIGS (virus-induced gene silencing). Plants with silenced CaMYB59 displayed not only compromised pepper immunity against RSI but also impaired tolerance to HTHH along with decreased hypersensitive response (HR). This impairment in defense function was fully linked with low induction of stress-linked genes like CaPO2, CaPR1, CaAcc and thermo-tolerance linked CaHSP24 as well as CaHsfB2a. Conversely, transient overexpression of CaMYB59 enhanced pepper immunity. This reveals that CaMYB59 positively regulated host defense against RSI and HTHH by means of HR like mimic cell death, HO production and up-regulation of defense as well as thermo-tolerance associated genes. These changes in attributes collectively confirm the role of CaMYB59 as a positive regulator of pepper immunity against . We recommend that such positive regulation of pepper defense is dynamically supported by phyto-hormone signaling and transcriptional web of defense genes. These integrated and interlinked events stabilize plant growth and survival under abiotic and biotic stresses.

中文翻译：

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CaMYB59 正向调控辣椒对青枯菌和高温胁迫的防御

我们对转录因子在辣椒针对攻击和高温高湿 (HTHH) 的免疫反应中的作用进行了功能评估。接触 。接种 (RSI) 和 HTHH 导致这种核定位 TF 的上调。通过 VIGS（病毒诱导基因沉默）进行 CaMYB59 功能丧失测定，证实了该 TF 的功能。CaMYB59 沉默的植物不仅表现出辣椒对 RSI 的免疫力受损，而且对 HTHH 的耐受性受损，同时过敏反应 (HR) 降低。这种防御功能受损与应激相关基因（如 CaPO2、CaPR1、CaAcc）和耐热性相关 C​​aHSP24 以及 CaHsfB2a 的低诱导完全相关。相反，CaMYB59 的短暂过度表达增强了辣椒的免疫力。这表明CaMYB59通过模拟细胞死亡、H2O产生和上调防御以及耐热相关基因等HR，正向调节宿主针对RSI和HTHH的防御。这些属性的变化共同证实了 CaMYB59 作为辣椒免疫的正调节剂的作用。我们建议，辣椒防御的这种正向调节是由植物激素信号传导和防御基因转录网络动态支持的。这些综合且相互关联的事件可稳定植物在非生物和生物胁迫下的生长和存活。