The nickel hyperaccumulator Bornmuellera tymphaea has been evaluated for use in agromining in Greece with very promising results. This study examines propagation of this species based on cuttings, which provides rapid multiplication and clonal propagation of genotypes with useful genetic traits and offers obvious advantages over propagation by seed. Apical cuttings, 10 cm long, obtained from plants in the field, were propagated on mist benches under greenhouse conditions. Four treatments based on the rooting medium were evaluated: (a) perlite, (b) perlite + peat 1:1 (v/v), (c) ultramafic soil, and (d) ultramafic soil + perlite 1:1 (v/v). Completely randomized blocks were used in a 4 × 2 factorial design, using 4 different propagation rooting media and 2 levels of auxins (with or without the use of indole‐3‐butyric acid [IBA]), with 3 replicates of 28 cuttings. The response to perlite medium was best in terms of rooting: 75% without IBA and 71.33% with IBA, and the dry weight of roots was 0.18 g and of shoots 0.34 g. Perlite + peat 1:1 (v/v) medium also resulted in rooting in more than 55% of cuttings whereas cuttings grown in ultramafic soil showed very low rooting (<27%). Based on the findings, B. tymphaea has an aptitude for vegetative propagation by cuttings with survival values and rooting greater than 70%. Perlite combined with the application of auxin (ΙΒΑ) is recommended.

中文翻译：

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Bornmuellera tymphaea（一种用于农业采矿的镍超积累植物）的插条繁殖：生根介质和生长素对茎插条的影响

镍超富集植物鼓膜博氏菌已在希腊对用于农业采矿进行了评估，并取得了非常有希望的结果。这项研究检查了该物种基于插条的繁殖，它提供了具有有用遗传性状的基因型的快速繁殖和克隆繁殖，并且比种子繁殖具有明显的优势。从田间植物获得的 10 厘米长的顶端插条在温室条件下的雾长凳上繁殖。评估了基于生根培养基的四种处理：（a）珍珠岩，（b）珍珠岩+泥炭1：1（v / v），（c）超镁铁质土壤，和（d）超镁铁质土壤+珍珠岩1：1（v /五）。完全随机区组采用 4 × 2 析因设计，使用 4 种不同的繁殖生根培养基和 2 种生长素水平（使用或不使用吲哚-3-丁酸 [IBA]），28 个插条进行 3 个重复。就生根而言，对珍珠岩培养基的反应最好：无IBA为75%，有IBA为71.33%，根干重为0.18 g，芽干重为0.34 g。珍珠岩 + 泥炭 1:1 (v/v) 培养基也导致超过 55% 的插条生根，而在超镁铁质土壤中生长的插条则显示出非常低的生根率 (<27%)。根据调查结果，乙。鼓室具有通过插条进行无性繁殖的能力，成活率和生根率大于70%。建议将珍珠岩与生长素（IΒA）结合使用。