Sampled ticks were screened for Crimean-Congo haemorrhagic fever virus (CCHFV) using an assay that targets the nucleoprotein gene region of the S segment, a conserved region of the CCHFV genome. Minimum infection rates of 0.34% and 0.10% were obtained when testing pools of Hyalomma rufipes and Amblyomma variegatum, respectively. Next-generation sequencing and phylogenetic analysis showed that the S and L segments of the CCHFV isolate clustered with those of similar isolates of genotype III. However, analysis of the M segment showed that reassortment had occurred, causing this segment to cluster with those of isolates of genotype I, providing the first evidence of such an occurrence in Ghana.

使用针对 S 片段核蛋白基因区域（CCHFV 基因组的保守区域）的检测方法，对蜱虫样本进行克里米亚-刚果出血热病毒 (CCHFV) 筛查。当测试红斑透明眼虫和花色斑眼眼虫的池时，分别获得了 0.34% 和 0.10% 的最低感染率。下一代测序和系统发育分析表明，CCHFV 分离株的 S 和 L 片段与基因型 III 的类似分离株聚集在一起。然而，对 M 片段的分析表明，发生了重配，导致该片段与基因型 I 的分离株聚集在一起，为加纳发生这种情况提供了第一个证据。