Research on protein hydrolysates has observed various properties and functionalities on ingredients depending on the type of hydrolysate. The objective of this study was to evaluate the effects of hydrolyzed chicken protein that was incorporated into diets on digestibility, gut health, skin and coat health, oxidative stress, and intestinal inflammation markers in healthy adult dogs. Five complete and balanced diets were manufactured: 1) CONd: 25% chicken meal diet; 2) 5% CLHd: 5% chicken liver and heart hydrolysate plus 20% chicken meal diet; 3) CLHd: 25% chicken liver and heart hydrolysate diet; 4) 5% CHd: 5% chicken hydrolysate plus 20% chicken meal diet; 5) CHd: 25% chicken hydrolysate diet. A replicated 5×5 Latin square design was used which included 10 neutered adult Beagles. Each of the 5 periods consisted of a 7-d washout time and a 28-d treatment period. All diets were well accepted by the dogs. Fecal butyrate concentration was higher while fecal isovalerate and total phenol/indole were lower in dogs fed CLHd than CONd (P < 0.05). Dogs fed CHd had higher fecal immunoglobulin A concentration when compared with CLHd (P < 0.05); however, both groups were comparable to the CONd. There was no difference among groups in serum cytokine concentrations, serum oxidative stress biomarkers, or skin and coat health analyses (P > 0.05). Fecal microbiota was shifted by CLHd with higher abundance in Ruminococcus gauvreauii group as well as lower Clostridium sensu stricto 1, Sutterella, Fusobacterium, and Bacteroides when compared with CONd (P < 0.05). There was also a difference in beta diversity of fecal microbiota between CLHd and CHd (P < 0.05). In conclusion, chicken protein hydrolysate could be incorporated into canine extruded diets as a comparable source of protein to traditional chicken meal. The test chicken protein hydrolysates showed the potential to support gut health by modulating immune response and microbiota; however, functional properties of protein hydrolysates are dependent on inclusion level and source.

中文翻译：

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水解蛋白对成年犬常量营养素消化率、粪便代谢物和微生物群、氧化应激和炎症生物标志物以及皮肤和皮毛质量的影响

对蛋白质水解产物的研究观察到，根据水解产物的类型，成分具有不同的特性和功能。本研究的目的是评估饮食中加入的水解鸡肉蛋白对健康成年犬的消化率、肠道健康、皮肤和皮毛健康、氧化应激和肠道炎症标志物的影响。制作了五种完整且均衡的饮食：1）CONd：25％鸡粉饮食；2) 5% CLHd：5%鸡肝和鸡心水解物加20%鸡粉日粮；3）CLHd：25%鸡肝和鸡心水解物日粮；4) 5% CHd：5%鸡肉水解物加20%鸡粉日粮；5) CHd：25%鸡水解物日粮。使用复制的 5×5 拉丁方设计，其中包括 10 只绝育的成年比格犬。5 个周期中的每个周期均包含 7 天的冲洗时间和 28 天的处理时间。所有饮食都被狗很好地接受。饲喂CLHd的狗的粪便丁酸盐浓度高于CONd，而粪便异戊酸和总苯酚/吲哚则低于CONd（P＜0.05）。与 CLHd 相比，饲喂 CHd 的狗粪便免疫球蛋白 A 浓度较高（P < 0.05）；然而，两组均与 CONd 具有可比性。血清细胞因子浓度、血清氧化应激生物标志物或皮肤和皮毛健康分析各组之间没有差异（P>0.05）。与 CONd 相比，CLHd 改变了粪便微生物群，高氏瘤胃球菌组的丰度较高，狭义梭状芽胞杆菌 1、Sutterella、梭杆菌和拟杆菌的丰度较低（P < 0.05）。CLHd 和 CHd 之间粪便微生物群的 β 多样性也存在差异（P < 0.05）。总之，鸡肉蛋白水解物可以作为与传统鸡肉粉相当的蛋白质来源纳入犬类膨化饲料中。测试的鸡肉蛋白水解物显示出通过调节免疫反应和微生物群来支持肠道健康的潜力；然而，蛋白质水解产物的功能特性取决于内含物水平和来源。