当前位置：
X-MOL 学术
›
Cancer Manage. Res.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
High Expression of WT1 and Low Expression of p53 in Archived Blocks of Children with Wilms Tumor in South Western Uganda
Cancer Management and Research ( IF 3.3 ) Pub Date : 2024-03-06 , DOI: 10.2147/cmar.s449982 Brian Ssenkumba , Barnabas Atwiine , Yekosani Mitala , Janet Adongo , Samuel Olowo , Rita Nabulya , Amnia Diaz Anaya , Raymond Atwine
Cancer Management and Research ( IF 3.3 ) Pub Date : 2024-03-06 , DOI: 10.2147/cmar.s449982 Brian Ssenkumba , Barnabas Atwiine , Yekosani Mitala , Janet Adongo , Samuel Olowo , Rita Nabulya , Amnia Diaz Anaya , Raymond Atwine
Purpose: The determination of the presence or absence of anaplasia in Wilms tumor is difficult sometimes creating diagnostic errors and is worsened by the use of neoadjuvant chemotherapy, which causes cellular alterations that may mimic anaplasia. This study described the histological features of Wilms tumor and their association with WT1 and p53 expression in archived specimens in South Western Uganda.
Patients and Methods: A series of 308 formalin-fixed paraffin-embedded tissue blocks belonging to 85 children were retrospectively recruited in the only public Histopathology laboratory in South Western Uganda. Rabbit monoclonal Anti-Wilms tumor protein antibody [(CAN-R9) IHC-56-2] ab89901 and rabbit monoclonal Anti-p53 antibody [E26] ab32389 were used to assess the expression of WT1 and p53, respectively. The expression of WT1 and p53 were reported as proportions, Chi-square was also performed to assess for associations and statistical significance was considered when the p-value was less than 0.05.
Results: The median age was 3.5 with an interquartile range of (2– 6) years. Mixed histology was the most common at 35.29% (95% CI:25.77– 46.14). Anaplasia was present in 5.88% (95% CI:2.44– 13.52) of the specimens. p53 and WT1 expressions were 13.0% (95% CI:7.25– 22.04), and 41.0% (95% CI: 31.11– 52.04), respectively.
Conclusion: Mixed-type histology is the most common histologic feature of Wilms tumor with high expression of WT1 and a low expression of p53 implying that these can be used routinely to confirm the diagnosis as well as anaplasia in South Western Uganda.
Keywords: children, Wilms tumor, p53, WT1, South Western Uganda
中文翻译：
乌干达西南部肾母细胞瘤儿童存档块中 WT1 高表达和 p53 低表达
目的：确定肾母细胞瘤是否存在退行性变很困难，有时会产生诊断错误，并且由于新辅助化疗的使用而使情况变得更糟，新辅助化疗会导致可能模仿退行性的细胞改变。这项研究描述了维尔姆斯瘤的组织学特征及其与乌干达西南部存档标本中 WT1 和 p53 表达的关系。
患者和方法：在乌干达西南部唯一的公共组织病理学实验室回顾性招募了属于 85 名儿童的一系列 308 个福尔马林固定石蜡包埋的组织块。兔单克隆抗Wilms肿瘤蛋白抗体[(CAN-R9) IHC-56-2] ab89901和兔单克隆抗p53抗体[E26] ab32389分别用于评估WT1和p53的表达。WT1和p53的表达以比例报告，还进行卡方评估关联性，并且当p值小于0.05时考虑统计显着性。
结果：中位年龄为 3.5 岁，四分位数范围为 (2-6) 岁。混合组织学最常见，占 35.29% (95% CI:25.77–46.14)。5.88% (95% CI:2.44–13.52) 的标本存在发育不全。p53 和 WT1 表达率分别为 13.0% (95% CI:7.25–22.04) 和 41.0% (95% CI: 31.11–52.04)。
结论：混合型组织学是乌干达西南部肾母细胞瘤最常见的组织学特征，WT1 高表达，p53 低表达，这意味着这些可以常规用于确诊和退行性变。
关键词：儿童、肾母细胞瘤、p53、WT1、乌干达西南部
更新日期：2024-03-06
Patients and Methods: A series of 308 formalin-fixed paraffin-embedded tissue blocks belonging to 85 children were retrospectively recruited in the only public Histopathology laboratory in South Western Uganda. Rabbit monoclonal Anti-Wilms tumor protein antibody [(CAN-R9) IHC-56-2] ab89901 and rabbit monoclonal Anti-p53 antibody [E26] ab32389 were used to assess the expression of WT1 and p53, respectively. The expression of WT1 and p53 were reported as proportions, Chi-square was also performed to assess for associations and statistical significance was considered when the p-value was less than 0.05.
Results: The median age was 3.5 with an interquartile range of (2– 6) years. Mixed histology was the most common at 35.29% (95% CI:25.77– 46.14). Anaplasia was present in 5.88% (95% CI:2.44– 13.52) of the specimens. p53 and WT1 expressions were 13.0% (95% CI:7.25– 22.04), and 41.0% (95% CI: 31.11– 52.04), respectively.
Conclusion: Mixed-type histology is the most common histologic feature of Wilms tumor with high expression of WT1 and a low expression of p53 implying that these can be used routinely to confirm the diagnosis as well as anaplasia in South Western Uganda.
Keywords: children, Wilms tumor, p53, WT1, South Western Uganda
中文翻译：
乌干达西南部肾母细胞瘤儿童存档块中 WT1 高表达和 p53 低表达
目的：确定肾母细胞瘤是否存在退行性变很困难，有时会产生诊断错误，并且由于新辅助化疗的使用而使情况变得更糟，新辅助化疗会导致可能模仿退行性的细胞改变。这项研究描述了维尔姆斯瘤的组织学特征及其与乌干达西南部存档标本中 WT1 和 p53 表达的关系。
患者和方法：在乌干达西南部唯一的公共组织病理学实验室回顾性招募了属于 85 名儿童的一系列 308 个福尔马林固定石蜡包埋的组织块。兔单克隆抗Wilms肿瘤蛋白抗体[(CAN-R9) IHC-56-2] ab89901和兔单克隆抗p53抗体[E26] ab32389分别用于评估WT1和p53的表达。WT1和p53的表达以比例报告，还进行卡方评估关联性，并且当p值小于0.05时考虑统计显着性。
结果：中位年龄为 3.5 岁，四分位数范围为 (2-6) 岁。混合组织学最常见，占 35.29% (95% CI:25.77–46.14)。5.88% (95% CI:2.44–13.52) 的标本存在发育不全。p53 和 WT1 表达率分别为 13.0% (95% CI:7.25–22.04) 和 41.0% (95% CI: 31.11–52.04)。
结论：混合型组织学是乌干达西南部肾母细胞瘤最常见的组织学特征，WT1 高表达，p53 低表达，这意味着这些可以常规用于确诊和退行性变。
关键词：儿童、肾母细胞瘤、p53、WT1、乌干达西南部
京公网安备 11010802027423号