Abstract

The present study highlights the effect of silver nitrate (AgNO₃) in the promotion of in vitro regeneration and morpho-structural growth and developments in Oxystelma esculentum (L.f.) Sm. The incorporation of AgNO₃ into the Murashige and Skoog (MS) medium resulted in a favorable enhancement of shoot proliferation. Among the different concentrations of AgNO₃ screened, 0.5 mg L⁻¹ AgNO₃ along with 0.5 mg L⁻¹ meta-Topolin (mT) and 0.25 mg L⁻¹ indole-3-acetic acid (IAA) stimulated the highest count, and length of shoots (143 shoots and 14.0 cm length) after 3 mo of incubation and the 3rd subculture. The in vitro–induced deformities in the tissues of the control stem and foliages were improved when the shoots were grown in AgNO₃-enriched medium. Silver nitrate increased the photosynthetic pigments and improved the formation of functional stomata. The AgNO₃-supplemented medium-derived shoots exhibited accelerated adventitious root induction (100% rhizogenesis, 21.0 roots per shoot) when the cut ends of shoots were treated with 200.0 mg L⁻¹ indole-3-butyric acid (IBA) for 5 min under greenhouse conditions. The incorporation of AgNO₃ with optimal plant growth regulators in the medium promoted overall growth. It prevented in vitro–induced morphological and anatomical anomalies in the leaf and stem, which considerably improved the greenhouse and in vivo survival of micropropagated plantlets.

中文翻译：

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硝酸银介导 Oxystelma esculentum (Lf) Sm 的微繁殖和微形态解剖结构的改善。

摘要

本研究强调了硝酸银 (AgNO ₃ ) 在促进Oxystelma esculentum (Lf) Sm体外再生和形态结构生长和发育中的作用。将AgNO ₃掺入Murashige 和Skoog (MS) 培养基中导致芽增殖的有利增强。在筛选的不同浓度的AgNO ₃中，0.5 mg L ^-1 AgNO ₃以及0.5 mg L ^-1间托普林（m T）和0.25 mg L ^-1吲哚-3-乙酸（IAA）刺激的计数最高，培养 3 个月和第 3 次继代培养后的芽长度（143 个芽和 14.0 厘米长）。_{当芽在富含AgNO 3}的培养基中生长时，对照茎和叶的组织中体外诱导的畸形得到改善。硝酸银增加光合色素并改善功能性气孔的形成。^{当芽的切端用200.0 mg L -1}吲哚-3-丁酸（IBA）处理5分钟时，添加AgNO ₃的培养基衍生芽表现出加速的不定根诱导（100％发根，每个芽21.0个根）在温室条件下。在培养基中加入AgNO _{3和最佳植物生长调节剂可促进整体生长。}它可以防止体外诱导的叶和茎的形态和解剖异常，从而显着改善微繁殖苗的温室和体内存活率。