Regions of low oxygen tension are common features of inflamed and infected tissues and provide physiologic selective pressure for the expansion of cells with enhanced hypoxia tolerance. The aim of this study was to investigate whether macrophages resistant to death induced by hypoxia were accompanied by functional alterations. A mouse macrophage cell line (J774 cells) was used to obtain subpopulations of death-resistant macrophages induced by long-term exposure to severe hypoxia (<1% O2). The results indicated that exposing J774 macrophages to periods of severe hypoxia results in the selection of cells with phenotypes associated with the modulation of heat-shock protein 70 kDa (HSP70) expression, tumor necrosis factor-α (TNF-α), and nitric oxide (NO) production and reduced susceptibility to parasite Leishmania infection. Thus, we suggest that hypoxia-selected macrophages may influence the outcome of inflammation and infection. Exp Biol Med 232:88–95, 2007

中文翻译：

---

缺氧选择后巨噬细胞的功能改变

低氧张力区域是发炎和感染组织的常见特征，并为细胞的扩张提供生理选择压力，增强缺氧耐受性。本研究的目的是调查巨噬细胞对缺氧诱导死亡的抵抗是否伴随着功能改变。使用小鼠巨噬细胞系（J774细胞）获得长期严重缺氧（<1%O2）诱导的抗死亡巨噬细胞亚群。2）。结果表明，将 J774 巨噬细胞暴露于严重缺氧时期会导致选择具有与热休克蛋白 70 kDa (HSP70) 表达、肿瘤坏死因子-α (TNF-α) 和一氧化氮调节相关的表型的细胞(NO) 的产生并降低对寄生虫利什曼原虫感染的易感性。因此，我们认为缺氧选择的巨噬细胞可能会影响炎症和感染的结果。实验生物医学 232:88–95, 2007