Molecular imprinting polymers (MIPs) are synthetic receptors as biomimetic materials for various applications ranging from sensing to separation and catalysis. However, currently existing MIPs are stuck to some of the issues including the longer preparation steps and poor performance. In this report, a facile and one-pot strategy by integrating the in-situ growth of magnetic nanoparticles and reversed phase microemulsion oriented molecularly imprinting strategy to develop magnetic molecular imprinted nanocomposites was proposed. Through self-assembling of the template, it brought up highly ordered and uniform arrangement of the imprinting structure, which offered faster adsorption kinetic as adsorption equilibrium was achived within 15 min, higher adsorption capacity (Q = 48.78 ± 1.54 μmol/g) and high affinity ( = 127.63 ± 9.66 μM) toward paradigm molecule-adenosine monophosphate (AMP) compared to the conventional bulk imprinting. The developed MIPs offered better affinity and superior specificity which allowed the specific enrichment toward targeted phosphorylated peptides from complex samples containing 100-fold more abundant interfering peptides. Interestingly, different types of MIPs can be developed which could targetly enrich the specific phosphorylated peptides for mass spectrometry analysis by simply switching the templates, and this strategy also successfully achieved imprinting of macromolecular peptides. Collectively, the approach showed broad applicability to target specific enrichment from metabolites to phosphorylated peptides and providing an alternative choice for selective recognition and analysis from complex biological systems.

中文翻译：

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模板的定向对接可提高修饰肽的印迹效率

分子印迹聚合物 (MIP) 是作为仿生材料的合成受体，适用于从传感到分离和催化等各种应用。然而，目前现有的 MIP 仍存在一些问题，包括准备步骤较长和性能较差。在本报告中，提出了一种简单的一锅策略，通过整合磁性纳米粒子的原位生长和反相微乳液定向分子印迹策略来开发磁性分子印迹纳米复合材料。通过模板的自组装，形成高度有序且均匀的印迹结构排列，从而提供更快的吸附动力学，在15分钟内达到吸附平衡，更高的吸附容量（Q=48.78±1.54μmol/g）和高吸附量。与传统的体印迹相比，对范例分子单磷酸腺苷 (AMP) 的亲和力 (= 127.63 ± 9.66 μM)。开发的 MIP 具有更好的亲和力和卓越的特异性，可以从含有丰富 100 倍干扰肽的复杂样品中特异性富集目标磷酸化肽。有趣的是，可以开发出不同类型的MIP，通过简单地切换模板即可靶向富集特定磷酸化肽用于质谱分析，并且该策略也成功实现了大分子肽的印迹。总的来说，该方法显示了从代谢物到磷酸化肽的靶向特异性富集的广泛适用性，并为复杂生物系统的选择性识别和分析提供了替代选择。