Zinc oxide nanostructures (ZnO NS) were fabricated in situ within a ternary hydrogel system composed of carboxymethyl cellulose-agarose-polyvinylpyrrolidone (CAP@ZnO TNCHs) by a one-pot method employing moist-heat solution casting. The percentages of CMC and ZnO NS were varied in the CAP hydrogel films and then they were investigated by different techniques, such as ATR/FTIR, TGA, XRD, XPS, and FE-SEM analysis. Furthermore, the mechanical properties, hydrophilicity, swelling, porosity, and antibacterial activity of the CAP@ZnO TNCHs were studied. In-vitro biocompatibility assays were performed with skin fibroblast (CCD-986sk) cells. In-vitro culture of CCD-986sk fibroblasts showed that the ZnO NS facilitated cell adhesion and proliferation. Furthermore, the application of CAP@ZnO TNCHs enhanced cellular interactions and physico-chemical, antibacterial bacterial, and biological performance relative to unmodified CAP hydrogels. Also, an in vivo wound healing study verified that the CAP@ZnO TNCHs promoted wound healing significantly within 18 days, an effect superior to that of unmodified CAP hydrogels. Hence, these newly developed cellulose-based ZnO TNCHs are promising materials for wound healing applications.

中文翻译：

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在羧甲基纤维素基三元水凝胶中原位制造 ZnO 纳米结构，用于伤口愈合应用

采用湿热溶液浇铸的一锅法，在由羧甲基纤维素-琼脂糖-聚乙烯吡咯烷酮（CAP@ZnO TNCHs）组成的三元水凝胶系统中原位制备了氧化锌纳米结构（ZnO NS）。 CAP 水凝胶薄膜中 CMC 和 ZnO NS 的百分比不同，然后通过不同的技术（例如 ATR/FTIR、TGA、XRD、XPS 和 FE-SEM 分析）对其进行研究。此外，还研究了CAP@ZnO TNCHs的机械性能、亲水性、膨胀性、孔隙率和抗菌活性。使用皮肤成纤维细胞 (CCD-986sk) 进行体外生物相容性测定。 CCD-986sk 成纤维细胞的体外培养表明，ZnO NS 促进细胞粘附和增殖。此外，相对于未修饰的 CAP 水凝胶，CAP@ZnO TNCH 的应用增强了细胞相互作用以及理化、抗菌和生物性能。此外，一项体内伤口愈合研究证实，CAP@ZnO TNCHs 在 18 天内显着促进伤口愈合，效果优于未修饰的 CAP 水凝胶。因此，这些新开发的纤维素基 ZnO TNCH 是用于伤口愈合应用的有前途的材料。