3D printing has revolutionized the manufacturing process of microanalytical devices by enabling the automated production of customized objects. This technology promises to become a fundamental tool, accelerating investigations in critical areas of health, food, and environmental sciences. This microfabrication technology can be easily disseminated among users to produce further and provide analytical data to an interconnected network towards the Internet of Things, as 3D printers enable automated, reproducible, low-cost, and easy fabrication of microanalytical devices in a single step. New functional materials are being investigated for one-step fabrication of highly complex 3D printed parts using photocurable resins. However, they are not yet widely used to fabricate microfluidic devices. This is likely the critical step towards easy and automated fabrication of sophisticated, complex, and functional 3D-printed microchips. Accordingly, this review covers recent advances in the development of 3D-printed microfluidic devices for point-of-care (POC) or bioanalytical applications such as nucleic acid amplification assays, immunoassays, cell and biomarker analysis and organs-on-a-chip. Finally, we discuss the future implications of this technology and highlight the challenges in researching and developing appropriate materials and manufacturing techniques to enable the production of 3D-printed microfluidic analytical devices in a single step.

中文翻译：

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生物分析应用3D打印微流控装置的最新成就和未来趋势回顾

3D 打印实现了定制物体的自动化生产，彻底改变了微分析设备的制造过程。这项技术有望成为一种基本工具，加速健康、食品和环境科学关键领域的研究。这种微加工技术可以很容易地在用户之间传播，以进一步生产并向物联网互连网络提供分析数据，因为 3D 打印机可以实现自动化、可重复、低成本且易于一步地制造微分析设备。人们正在研究新型功能材料，以使用光固化树脂一步制造高度复杂的 3D 打印零件。然而，它们尚未广泛用于制造微流体装置。这可能是轻松自动化制造精密、复杂和功能性 3D 打印微芯片的关键一步。因此，本综述涵盖了用于即时护理 (POC) 或生物分析应用（例如核酸扩增测定、免疫测定、细胞和生物标志物分析以及器官芯片）的 3D 打印微流体设备开发的最新进展。最后，我们讨论了该技术的未来影响，并强调了研究和开发适当的材料和制造技术以实现一步生产 3D 打印微流体分析设备所面临的挑战。