Nitrite has been involved in many food processing techniques and its excessive consumption is closely related to the development of different diseases. Therefore, highly sensitive detection of nitrite is significant to ensure food safety. This study presents a simple and novel strategy for the highly sensitive detection of nitrite in food using paper-based analytical devices (PADs). In this proposed strategy, the nitrite present in the sample undergoes efficient diazotization when initially mixed with sulfanilamide solution before reacting with N-(1-naphthyl) ethylenediamine dihydrochloride (NED) coated on the detection region of the PAD, leading to the maximum production of colored azo compounds. Specifically, within the concentration range of 0.1–20 mg/L, the LOD and LOQ for the nitrite assay using the premixing strategy are determined as 0.053 mg/L and 0.18 mg/L, respectively which significantly surpass the corresponding values of 0.18 mg/L (LOD) and 0.61 mg/L (LOQ) achieved with the regular Griess reagent analysis. The study highlights the critical importance of the premixing strategy in nitrite detection. Under optimized conditions, the strategy demonstrates an excellent limit of detection (LOD) and limit of quantification (LOQ) for nitrite detection in eight different meat samples. In addition to its high precision, the strategy is applicable in the field of nitrite analysis. This strategy could facilitate rapid and cost-effective nitrite analysis in real food samples, ensuring food safety and quality analysis.

中文翻译：

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一种用于在纸基分析装置上高灵敏度检测亚硝酸盐的新型预混合策略

亚硝酸盐已参与多种食品加工工艺，其过量摄入与多种疾病的发生密切相关。因此，亚硝酸盐的高灵敏度检测对于保障食品安全具有重要意义。本研究提出了一种简单而新颖的策略，可使用纸基分析装置 (PAD) 对食品中的亚硝酸盐进行高灵敏度检测。在该策略中，样品中存在的亚硝酸盐最初与磺胺溶液混合，然后与涂覆在 PAD 检测区域上的 N-(1-萘基)乙二胺二盐酸盐 (NED) 反应，从而发生有效的重氮化，从而最大程度地产生亚硝酸盐。有色偶氮化合物。具体而言，在0.1–20 mg/L的浓度范围内，采用预混合策略的亚硝酸盐测定的LOD和LOQ分别确定为0.053 mg/L和0.18 mg/L，显着超过了相应值0.18 mg/L。通过常规 Griess 试剂分析获得 L (LOD) 和 0.61 mg/L (LOQ)。该研究强调了预混合策略在亚硝酸盐检测中的至关重要性。在优化条件下，该策略在八种不同肉类样品中的亚硝酸盐检测中表现出出色的检测限 (LOD) 和定量限 (LOQ)。除了精度高之外，该策略还适用于亚硝酸盐分析领域。该策略可以促进实际食品样品中快速且经济高效的亚硝酸盐分析，确保食品安全和质量分析。