Cancer as a leading cause of premature death worldwide has become a major threat to human health due to the high incidence and mortality. Monitoring tumor markers are reliable and significantly important for early detection of cancers. In complex biological systems, it is of great urgency but still remains challenging to conceive a fluorescent probe with multiple tumor markers detection property. Hydrogen sulfide (HS) and pH are two target biomarkers for diagnosis of early cancer. The preparation of a novel probe with HS and pH dual detection functions is highly anticipated. Herein, a novel sequential detection probe for HS and pH has been developed. In this system, HPQ (2-(2 -hydroxyphenyl)-4(3H)-quinazolinone) structure combined with triphenylamine is applied as the fluorophore, and 2, 4-dinitrophenylsulfonyl group is used as the recognition group. In the presence of HS, is transformed into product which shows fluorescence enhancement (29-fold) at 525 nm in less than 4 min and further displays repeatable acid-base responsive ability. is able to sequentially response to HS and pH in living cells and does not react directly with pH. Owing to the low cytotoxicity, is able to detect exogenous and endogenous HS in colon cancer cells and mice, monitor HS in inflammation model and in foodstuffs. As the environment changes from acidic to alkaline, the fluorescence intensity ratio (I/I) of product changes remarkably, illustrating the ratiometric fluorescent responsiveness to pH. A multifunctional fluorescent probe for sequential detection of HS and pH is synthesized. Probe realizes the monitoring of dynamic changes in intracellular pH and displays prospective application in security printing. We expect that our work could offer an important guidance on the development of multifunctional fluorescent probes for visualizing HS and pH in biology and environment.

中文翻译：

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一种多功能荧光探针，用于连续检测食品、活细胞和小鼠中的硫化氢和 pH 值

癌症作为全球过早死亡的主要原因，由于其高发病率和死亡率，已成为人类健康的主要威胁。监测肿瘤标志物对于癌症的早期检测是可靠的并且非常重要。在复杂的生物系统中，构思具有多种肿瘤标志物检测特性的荧光探针非常紧迫，但仍然具有挑战性。硫化氢 (HS) 和 pH 是诊断早期癌症的两个目标生物标志物。具有HS和pH双重检测功能的新型探针的制备备受期待。在此，开发了一种新型的 H2S 和 pH 连续检测探针。该体系以HPQ(2-(2-羟基苯基)-4(3H)-喹唑啉酮)结构与三苯胺结合作为荧光基团,2,4-二硝基苯磺酰基作为识别基团。在 HS 存在下，转化为产物，在 4 分钟内显示出 525 nm 处的荧光增强（29 倍），并进一步显示出可重复的酸碱响应能力。能够顺序响应活细胞中的 HS 和 pH，但不直接与 pH 发生反应。由于细胞毒性低，能够检测结肠癌细胞和小鼠中的外源性和内源性 HS，监测炎症模型和食品中的 HS。随着环境从酸性变为碱性，产品的荧光强度比 (I/I) 发生显着变化，说明了比率荧光对 pH 的响应性。合成了一种用于连续检测 HS 和 pH 的多功能荧光探针。探针实现了细胞内pH动态变化的监测，展示了在防伪印刷中的应用前景。我们希望我们的工作能够为开发用于可视化生物学和环境中 H2S 和 pH 的多功能荧光探针提供重要指导。