In health assessment and personalized medical services, accurate detection of biological markers such as dopamine (DA) and uric acid (UA) in sweat is crucial for providing valuable physiological information. However, there are challenges in detecting sweat biomarkers due to their low concentrations, variations in sweat yield among individuals, and the need for efficient sweat collection. We synthesized CuNi-MOF@rGO as a high-activity electrocatalyst and investigated its feasibility and electrochemical mechanism for simultaneously detecting low-concentration biomarkers UA and DA. Interaction between the non-coordinating carboxylate group and the sample produces effective separation signals for DA and UA. The wearable biomimetic biosensor has a wide linear range of 1–500 μM, with a detection limit of 9.41 μM and sensitivity of 0.019 μA μM cm for DA, and 10–1000 μM, with a detection limit of 9.09 μM and sensitivity of 0.026 μA μM cm for UA. Thus, our sensor performs excellently in detecting low-concentration biomarkers. To improve sweat collection, we designed a microfluidic-controlled device with hydrophilic modification in the microchannel. Experimental results show optimal ink flow at 2% concentration. Overall, we developed an innovative and highly active electrocatalyst, successfully enabling simultaneous detection of low-concentration biomarkers UA and DA. This study provides a strategy for sweat analysis and health monitoring. Moreover, the sensor also showed good performance in detecting real sweat samples. This study has shown great potential in future advances in sweat analysis and health monitoring.

中文翻译：

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CuNi-MOF@rGO修饰的可穿戴柔性电化学生物传感器可同时检测汗液中的尿酸和多巴胺

在健康评估和个性化医疗服务中，准确检测汗液中的多巴胺（DA）和尿酸（UA）等生物标志物对于提供有价值的生理信息至关重要。然而，由于汗液生物标志物浓度低、个体出汗量存在差异以及需要有效收集汗液，检测汗液生物标志物存在挑战。我们合成了 CuNi-MOF@rGO 作为高活性电催化剂，并研究了其同时检测低浓度生物标志物 UA 和 DA 的可行性和电化学机制。非配位羧酸根基团与样品之间的相互作用产生 DA 和 UA 的有效分离信号。该可穿戴仿生生物传感器具有较宽的线性范围：1-500 μM，检测限为 9.41 μM，灵敏度为 0.019 μA μM cm（DA）；10-1000 μM，检测限为 9.09 μM，灵敏度为 0.026 μA μM cm 为 UA。因此，我们的传感器在检测低浓度生物标志物方面表现出色。为了改善汗液收集，我们设计了一种在微通道中进行亲水修饰的微流体控制装置。实验结果表明，浓度为 2% 时墨水流动性最佳。总体而言，我们开发了一种创新且高活性的电催化剂，成功地实现了低浓度生物标志物 UA 和 DA 的同时检测。这项研究提供了汗液分析和健康监测的策略。此外，该传感器在检测真实汗液样本方面也表现出了良好的性能。这项研究显示了汗液分析和健康监测未来发展的巨大潜力。