Diabetic foot ulcers (DFUs) are one of the most serious and devastating complication of diabetes, manifesting as foot ulcers and impaired wound healing in patients with diabetes mellitus. To solve this problem, sulfated hyaluronic acid (SHA)/collagen-based nanofibrous biomimetic skins was developed and used to promote the diabetic wound healing and skin remodeling. First, SHA was successfully synthetized using chemical sulfation and incorporated into collagen (COL) matrix for preparing the SHA/COL hybrid nanofiber skins. The polyurethane (PU) was added into those hybrid scaffolds to make up the insufficient mechanical properties of SHA/COL nanofibers, the morphology, surface properties and degradation rate of hybrid nanofibers, as well as cell responses upon the nanofibrous scaffolds were studied to evaluate their potential for skin reconstruction. The results demonstrated that the SHA/COL, SHA/HA/COL hybrid nanofiber skins were stimulatory of cell behaviors, including a high proliferation rate and maintaining normal phenotypes of specific cells. Notably, SHA/COL and SHA/HA/COL hybrid nanofibers exhibited a significantly accelerated wound healing and a high skin remodeling effect in diabetic mice compared with the control group. Overall, SHA/COL-based hybrid scaffolds are promising candidates as biomimetic hybrid nanofiber skin for accelerating diabetic wound healing.

中文翻译：

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用于糖尿病伤口愈合的硫酸化透明质酸/胶原基仿生混合纳米纤维皮肤：开发和初步评估

糖尿病足溃疡（DFU）是糖尿病最严重和最具破坏性的并发症之一，表现为糖尿病患者的足部溃疡和伤口愈合受损。为了解决这个问题，开发了硫酸化透明质酸（SHA）/胶原基纳米纤维仿生皮肤，并将其用于促进糖尿病伤口愈合和皮肤重塑。首先，利用化学硫酸化成功合成了 SHA，并将其掺入胶原蛋白（COL）基质中，制备了 SHA/COL 混合纳米纤维皮。将聚氨酯（PU）添加到这些杂化支架中，以弥补SHA/COL纳米纤维机械性能的不足，研究杂化纳米纤维的形貌、表面性能和降解率，以及纳米纤维支架上的细胞反应，以评估其性能皮肤重建的潜力。结果表明，SHA/COL、SHA/HA/COL 混合纳米纤维皮可刺激细胞行为，包括高增殖率和维持特定细胞的正常表型。值得注意的是，与对照组相比，SHA/COL 和 SHA/HA/COL 混合纳米纤维在糖尿病小鼠中表现出显着加速的伤口愈合和较高的皮肤重塑效果。总体而言，基于 SHA/COL 的混合支架是作为仿生混合纳米纤维皮肤加速糖尿病伤口愈合的有希望的候选者。