In this work, an effective competitive-type electrochemiluminescence (ECL) immunosensor was constructed for zearalenone determination by using Zr-MOF nanoplates as the ECL luminophore and Au@MoS nanoflowers as the substrate material. Zr-MOF have an ultra-thin sheet-like structure that accelerates the transfer of electrons, ions and co-reactant intermediates, which exhibited strong and stable anodic luminescence. The three-dimensional Au@MoS nanoflowers would form a thin film modification layer on the glassy carbon electrode (GCE). And its good electrical conductivity and higher specific surface area utilization further improving the sensitivity of the ECL immunosensor. Under the optimized conditions, the proposed immunosensor exhibited satisfactory stability, sensitivity and accuracy, and its ECL signal was proportional to the logarithm of ZEN concentration (0.0001–100 ng/mL) and the limit of detection (LOD) was 0.034 pg/mL. In addition, the results of recovery experiment acquired for wheat flour and pig urine samples further proved the feasibility of the immunosensor for the detection of real samples, indicating its potential for ultrasensitive detection of ZEN.

中文翻译：

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基于 Zr-MOF 纳米板和 Au@MoS2 纳米花电化学发光免疫分析的玉米赤霉烯酮超灵敏检测

本工作以Zr-MOF纳米板为ECL发光体，Au@MoS纳米花为基质材料，构建了一种有效的竞争型电化学发光（ECL）免疫传感器，用于玉米赤霉烯酮的测定。 Zr-MOF具有超薄片状结构，可加速电子、离子和共反应中间体的转移，表现出强而稳定的阳极发光。三维 Au@MoS 纳米花将在玻碳电极（GCE）上形成薄膜修饰层。其良好的导电性和较高的比表面积利用率进一步提高了ECL免疫传感器的灵敏度。在优化条件下，所提出的免疫传感器表现出令人满意的稳定性、灵敏度和准确性，其ECL信号与ZEN浓度的对数（0.0001-100 ng/mL）成正比，检测限（LOD）为0.034 pg/mL。此外，小麦粉和猪尿样品的回收率实验结果进一步证明了免疫传感器检测真实样品的可行性，表明其超灵敏检测ZEN的潜力。